Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18585 and PS417_18590 are separated by 53 nucleotides PS417_18590 and PS417_18595 are separated by 162 nucleotides PS417_18595 and PS417_18600 are separated by 10 nucleotides
PS417_18585: PS417_18585 - 3-isopropylmalate dehydrogenase, at 4,036,729 to 4,037,811
_18585
PS417_18590: PS417_18590 - SAM-dependent methyltransferase, at 4,037,865 to 4,038,629
_18590
PS417_18595: PS417_18595 - 3-isopropylmalate dehydratase, at 4,038,792 to 4,039,436
_18595
PS417_18600: PS417_18600 - isopropylmalate isomerase, at 4,039,447 to 4,040,865
_18600
Position (kb) 4038
4039
4040 Strain fitness (log2 ratio) -1
0
1 at 4037.889 kb on + strand at 4037.889 kb on + strand at 4038.022 kb on + strand, within PS417_18590 at 4038.022 kb on + strand, within PS417_18590 at 4038.050 kb on + strand, within PS417_18590 at 4038.154 kb on + strand, within PS417_18590 at 4038.154 kb on + strand, within PS417_18590 at 4038.155 kb on - strand, within PS417_18590 at 4038.155 kb on - strand, within PS417_18590 at 4038.783 kb on + strand at 4038.787 kb on + strand at 4038.937 kb on + strand, within PS417_18595 at 4039.102 kb on + strand, within PS417_18595 at 4039.102 kb on + strand, within PS417_18595 at 4039.279 kb on + strand, within PS417_18595 at 4039.878 kb on - strand, within PS417_18600 at 4039.878 kb on - strand, within PS417_18600
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1 remove 4,037,889 + +0.2 4,037,889 + -0.3 4,038,022 + PS417_18590 0.21 -0.1 4,038,022 + PS417_18590 0.21 +0.1 4,038,050 + PS417_18590 0.24 +0.6 4,038,154 + PS417_18590 0.38 -0.1 4,038,154 + PS417_18590 0.38 +0.4 4,038,155 - PS417_18590 0.38 -0.1 4,038,155 - PS417_18590 0.38 -0.3 4,038,783 + -0.1 4,038,787 + +0.4 4,038,937 + PS417_18595 0.22 -0.7 4,039,102 + PS417_18595 0.48 +0.3 4,039,102 + PS417_18595 0.48 +0.5 4,039,279 + PS417_18595 0.76 -0.0 4,039,878 - PS417_18600 0.30 -0.2 4,039,878 - PS417_18600 0.30 +0.1
Or see this region's nucleotide sequence