Strain Fitness in Pseudomonas simiae WCS417 around PS417_00150

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_00145 and PS417_00150 are separated by 31 nucleotidesPS417_00150 and PS417_00155 are separated by 96 nucleotides PS417_00145: PS417_00145 - LysR family transcriptional regulator, at 31,569 to 32,519 _00145 PS417_00150: PS417_00150 - 4,5-dioxygenase, at 32,551 to 32,880 _00150 PS417_00155: PS417_00155 - 2-dehydro-3-deoxygalactonokinase, at 32,977 to 33,936 _00155 Position (kb) 32 33Strain fitness (log2 ratio) -1 0 1at 31.925 kb on + strand, within PS417_00145at 31.948 kb on + strand, within PS417_00145at 31.948 kb on + strand, within PS417_00145at 31.948 kb on + strand, within PS417_00145at 31.948 kb on + strand, within PS417_00145at 31.948 kb on + strand, within PS417_00145at 31.948 kb on + strand, within PS417_00145at 31.948 kb on + strand, within PS417_00145at 31.949 kb on - strand, within PS417_00145at 31.949 kb on - strand, within PS417_00145at 32.256 kb on + strand, within PS417_00145at 32.256 kb on + strand, within PS417_00145at 32.256 kb on + strand, within PS417_00145at 32.256 kb on + strand, within PS417_00145at 32.256 kb on + strand, within PS417_00145at 32.257 kb on - strand, within PS417_00145at 32.257 kb on - strand, within PS417_00145at 32.391 kb on + strand, within PS417_00145at 32.391 kb on + strand, within PS417_00145at 32.391 kb on + strand, within PS417_00145at 32.392 kb on - strand, within PS417_00145at 32.518 kb on - strandat 32.518 kb on - strandat 32.559 kb on + strandat 32.559 kb on + strandat 32.559 kb on + strandat 32.559 kb on + strandat 32.560 kb on - strandat 32.581 kb on - strandat 32.581 kb on - strandat 32.584 kb on + strand, within PS417_00150at 32.584 kb on + strand, within PS417_00150at 32.585 kb on - strand, within PS417_00150at 32.585 kb on - strand, within PS417_00150at 32.728 kb on + strand, within PS417_00150at 32.728 kb on + strand, within PS417_00150at 32.728 kb on + strand, within PS417_00150at 32.729 kb on - strand, within PS417_00150at 32.729 kb on - strand, within PS417_00150at 32.828 kb on - strandat 32.828 kb on - strand, within PS417_00150at 32.878 kb on + strandat 32.913 kb on - strandat 32.934 kb on + strandat 32.934 kb on + strandat 32.935 kb on - strandat 32.940 kb on + strandat 32.942 kb on + strandat 33.028 kb on + strandat 33.028 kb on + strandat 33.029 kb on - strandat 33.030 kb on + strandat 33.297 kb on + strand, within PS417_00155at 33.298 kb on - strand, within PS417_00155at 33.483 kb on + strand, within PS417_00155at 33.499 kb on - strand, within PS417_00155at 33.713 kb on - strand, within PS417_00155

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
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31,925 + PS417_00145 0.37 -0.2
31,948 + PS417_00145 0.40 +0.7
31,948 + PS417_00145 0.40 -0.1
31,948 + PS417_00145 0.40 +0.1
31,948 + PS417_00145 0.40 -0.3
31,948 + PS417_00145 0.40 +0.6
31,948 + PS417_00145 0.40 -0.1
31,948 + PS417_00145 0.40 -0.6
31,949 - PS417_00145 0.40 +0.3
31,949 - PS417_00145 0.40 -0.4
32,256 + PS417_00145 0.72 +0.4
32,256 + PS417_00145 0.72 +0.2
32,256 + PS417_00145 0.72 -0.0
32,256 + PS417_00145 0.72 -0.6
32,256 + PS417_00145 0.72 +0.1
32,257 - PS417_00145 0.72 -0.3
32,257 - PS417_00145 0.72 +0.0
32,391 + PS417_00145 0.86 -0.0
32,391 + PS417_00145 0.86 -0.3
32,391 + PS417_00145 0.86 -0.3
32,392 - PS417_00145 0.87 -0.2
32,518 - -0.8
32,518 - -0.2
32,559 + -0.9
32,559 + -0.1
32,559 + -0.1
32,559 + -0.0
32,560 - -0.2
32,581 - -1.3
32,581 - +0.3
32,584 + PS417_00150 0.10 -0.1
32,584 + PS417_00150 0.10 -0.4
32,585 - PS417_00150 0.10 -0.0
32,585 - PS417_00150 0.10 +0.4
32,728 + PS417_00150 0.54 +0.8
32,728 + PS417_00150 0.54 -0.4
32,728 + PS417_00150 0.54 +0.2
32,729 - PS417_00150 0.54 +0.4
32,729 - PS417_00150 0.54 -0.2
32,828 - -0.5
32,828 - PS417_00150 0.84 +0.5
32,878 + +0.1
32,913 - -0.0
32,934 + -0.0
32,934 + -0.2
32,935 - +0.4
32,940 + -0.8
32,942 + -0.1
33,028 + +0.9
33,028 + -0.0
33,029 - -0.2
33,030 + -0.4
33,297 + PS417_00155 0.33 +1.6
33,298 - PS417_00155 0.33 +0.2
33,483 + PS417_00155 0.53 -0.1
33,499 - PS417_00155 0.54 +0.7
33,713 - PS417_00155 0.77 -0.1

Or see this region's nucleotide sequence