Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27680 and PS417_27685 are separated by 133 nucleotides PS417_27685 and PS417_27690 are separated by 169 nucleotides PS417_27690 and PS417_27695 are separated by 116 nucleotides
PS417_27680: PS417_27680 - acetyl-CoA hydrolase, at 5,970,963 to 5,972,885
_27680
PS417_27685: PS417_27685 - cytochrome C, at 5,973,019 to 5,973,444
_27685
PS417_27690: PS417_27690 - XRE family transcriptional regulator, at 5,973,614 to 5,974,162
_27690
PS417_27695: PS417_27695 - alanine racemase, at 5,974,279 to 5,975,352
_27695
Position (kb)
5973
5974
5975 Strain fitness (log2 ratio)
-1
0
1 at 5972.722 kb on + strand at 5972.723 kb on - strand at 5972.993 kb on + strand at 5973.467 kb on - strand at 5973.541 kb on + strand at 5973.541 kb on + strand at 5973.546 kb on - strand at 5973.616 kb on - strand at 5973.706 kb on - strand, within PS417_27690 at 5973.706 kb on - strand, within PS417_27690 at 5973.914 kb on - strand, within PS417_27690 at 5973.914 kb on - strand, within PS417_27690 at 5973.936 kb on + strand, within PS417_27690 at 5973.936 kb on + strand, within PS417_27690 at 5973.997 kb on + strand, within PS417_27690 at 5973.997 kb on + strand, within PS417_27690 at 5973.998 kb on - strand, within PS417_27690 at 5974.130 kb on - strand at 5974.195 kb on + strand at 5974.196 kb on - strand at 5974.232 kb on - strand at 5974.232 kb on - strand at 5974.247 kb on - strand at 5974.292 kb on + strand at 5974.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 5,972,722 + -0.2 5,972,723 - -0.2 5,972,993 + -0.4 5,973,467 - +0.1 5,973,541 + -0.1 5,973,541 + +0.0 5,973,546 - -0.3 5,973,616 - -0.2 5,973,706 - PS417_27690 0.17 +0.4 5,973,706 - PS417_27690 0.17 -0.5 5,973,914 - PS417_27690 0.55 -0.6 5,973,914 - PS417_27690 0.55 -0.8 5,973,936 + PS417_27690 0.59 -0.3 5,973,936 + PS417_27690 0.59 +0.1 5,973,997 + PS417_27690 0.70 -0.5 5,973,997 + PS417_27690 0.70 -0.7 5,973,998 - PS417_27690 0.70 -1.7 5,974,130 - +0.0 5,974,195 + -0.5 5,974,196 - +0.2 5,974,232 - +0.8 5,974,232 - -0.3 5,974,247 - +1.0 5,974,292 + -0.2 5,974,293 - -1.1
Or see this region's nucleotide sequence