Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27470 and PS417_27475 are separated by 88 nucleotides PS417_27475 and PS417_27480 overlap by 1 nucleotides PS417_27480 and PS417_27485 are separated by 50 nucleotides PS417_27485 and PS417_27490 are separated by 76 nucleotides
PS417_27470: PS417_27470 - threonine transporter RhtB, at 5,929,106 to 5,929,738
_27470
PS417_27475: PS417_27475 - membrane protein, at 5,929,827 to 5,930,399
_27475
PS417_27480: PS417_27480 - ABC transporter ATP-binding protein, at 5,930,399 to 5,932,309
_27480
PS417_27485: PS417_27485 - hypothetical protein, at 5,932,360 to 5,932,827
_27485
PS417_27490: PS417_27490 - hypothetical protein, at 5,932,904 to 5,933,443
_27490
Position (kb) 5930
5931
5932
5933 Strain fitness (log2 ratio) -1
0
1 at 5929.485 kb on + strand, within PS417_27470 at 5929.486 kb on - strand, within PS417_27470 at 5929.521 kb on + strand, within PS417_27470 at 5929.522 kb on - strand, within PS417_27470 at 5929.522 kb on - strand, within PS417_27470 at 5929.522 kb on - strand, within PS417_27470 at 5929.522 kb on - strand, within PS417_27470 at 5929.620 kb on + strand, within PS417_27470 at 5929.639 kb on - strand, within PS417_27470 at 5929.726 kb on + strand at 5929.778 kb on + strand at 5929.779 kb on - strand at 5929.805 kb on - strand at 5929.813 kb on - strand at 5929.853 kb on + strand at 5929.955 kb on - strand, within PS417_27475 at 5930.311 kb on + strand, within PS417_27475 at 5930.484 kb on - strand at 5930.871 kb on - strand, within PS417_27480 at 5931.175 kb on - strand, within PS417_27480 at 5931.175 kb on - strand, within PS417_27480 at 5931.187 kb on + strand, within PS417_27480 at 5931.525 kb on + strand, within PS417_27480 at 5931.526 kb on - strand, within PS417_27480 at 5931.618 kb on + strand, within PS417_27480 at 5931.630 kb on + strand at 5931.630 kb on + strand, within PS417_27480 at 5931.630 kb on + strand, within PS417_27480 at 5931.726 kb on + strand, within PS417_27480 at 5931.895 kb on + strand, within PS417_27480 at 5931.942 kb on + strand at 5932.284 kb on - strand at 5932.289 kb on + strand at 5932.290 kb on - strand at 5932.290 kb on - strand at 5932.310 kb on - strand at 5932.326 kb on - strand at 5932.359 kb on + strand at 5932.359 kb on + strand at 5932.359 kb on + strand at 5932.359 kb on + strand at 5932.360 kb on - strand at 5932.360 kb on - strand at 5932.360 kb on - strand at 5932.396 kb on + strand at 5932.396 kb on + strand at 5932.396 kb on + strand at 5932.397 kb on - strand at 5932.571 kb on + strand, within PS417_27485 at 5932.572 kb on - strand, within PS417_27485 at 5932.581 kb on - strand, within PS417_27485 at 5932.581 kb on - strand, within PS417_27485 at 5932.826 kb on - strand at 5933.279 kb on + strand, within PS417_27490 at 5933.280 kb on - strand, within PS417_27490
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 5,929,485 + PS417_27470 0.60 -0.3 5,929,486 - PS417_27470 0.60 +0.0 5,929,521 + PS417_27470 0.66 -0.3 5,929,522 - PS417_27470 0.66 -0.1 5,929,522 - PS417_27470 0.66 -0.9 5,929,522 - PS417_27470 0.66 +0.9 5,929,522 - PS417_27470 0.66 -0.1 5,929,620 + PS417_27470 0.81 +0.1 5,929,639 - PS417_27470 0.84 +1.5 5,929,726 + -0.2 5,929,778 + +0.2 5,929,779 - +0.0 5,929,805 - +0.1 5,929,813 - +0.6 5,929,853 + +0.4 5,929,955 - PS417_27475 0.22 -0.5 5,930,311 + PS417_27475 0.84 -0.0 5,930,484 - -0.3 5,930,871 - PS417_27480 0.25 -0.4 5,931,175 - PS417_27480 0.41 -0.3 5,931,175 - PS417_27480 0.41 -0.1 5,931,187 + PS417_27480 0.41 +0.1 5,931,525 + PS417_27480 0.59 +0.5 5,931,526 - PS417_27480 0.59 -0.9 5,931,618 + PS417_27480 0.64 +0.8 5,931,630 + +0.1 5,931,630 + PS417_27480 0.64 +0.3 5,931,630 + PS417_27480 0.64 -0.4 5,931,726 + PS417_27480 0.69 -0.5 5,931,895 + PS417_27480 0.78 +0.9 5,931,942 + -0.2 5,932,284 - -0.6 5,932,289 + -0.4 5,932,290 - -1.0 5,932,290 - +0.2 5,932,310 - -0.4 5,932,326 - +0.2 5,932,359 + +1.0 5,932,359 + +0.1 5,932,359 + -0.4 5,932,359 + -0.7 5,932,360 - -0.3 5,932,360 - +0.2 5,932,360 - -0.1 5,932,396 + +0.3 5,932,396 + +0.1 5,932,396 + -1.2 5,932,397 - +0.1 5,932,571 + PS417_27485 0.45 +0.4 5,932,572 - PS417_27485 0.45 +0.5 5,932,581 - PS417_27485 0.47 +0.7 5,932,581 - PS417_27485 0.47 +0.1 5,932,826 - -0.6 5,933,279 + PS417_27490 0.69 +0.2 5,933,280 - PS417_27490 0.70 -0.3
Or see this region's nucleotide sequence