Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25980 and PS417_25985 are separated by 106 nucleotides PS417_25985 and PS417_25990 overlap by 4 nucleotides PS417_25990 and PS417_25995 overlap by 8 nucleotides PS417_25995 and PS417_26000 overlap by 8 nucleotides
PS417_25980: PS417_25980 - pyrroloquinoline quinone biosynthesis protein PqqE, at 5,614,742 to 5,615,023
_25980
PS417_25985: PS417_25985 - dethiobiotin synthetase, at 5,615,130 to 5,615,810
_25985
PS417_25990: PS417_25990 - malonyl-CoA O-methyltransferase, at 5,615,807 to 5,616,619
_25990
PS417_25995: PS417_25995 - transporter, at 5,616,612 to 5,617,343
_25995
PS417_26000: PS417_26000 - 8-amino-7-oxononanoate synthase, at 5,617,336 to 5,618,517
_26000
Position (kb) 5615
5616
5617 Strain fitness (log2 ratio) -1
0
1 at 5615.028 kb on + strand at 5615.029 kb on - strand at 5615.156 kb on - strand at 5615.279 kb on + strand, within PS417_25985 at 5615.793 kb on - strand at 5615.793 kb on - strand at 5615.798 kb on - strand at 5615.811 kb on - strand at 5615.833 kb on - strand at 5615.833 kb on - strand at 5615.845 kb on - strand at 5615.951 kb on - strand, within PS417_25990 at 5616.008 kb on + strand, within PS417_25990 at 5616.008 kb on + strand, within PS417_25990 at 5616.008 kb on + strand, within PS417_25990 at 5616.008 kb on + strand, within PS417_25990 at 5616.009 kb on - strand, within PS417_25990 at 5616.009 kb on - strand, within PS417_25990 at 5616.070 kb on - strand, within PS417_25990 at 5616.657 kb on + strand at 5617.300 kb on - strand at 5617.327 kb on - strand at 5617.327 kb on - strand at 5617.394 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 5,615,028 + -0.1 5,615,029 - +0.1 5,615,156 - -0.3 5,615,279 + PS417_25985 0.22 -0.0 5,615,793 - +0.1 5,615,793 - +0.6 5,615,798 - +0.4 5,615,811 - +0.1 5,615,833 - -0.2 5,615,833 - -0.8 5,615,845 - -0.2 5,615,951 - PS417_25990 0.18 -0.2 5,616,008 + PS417_25990 0.25 -0.5 5,616,008 + PS417_25990 0.25 -0.5 5,616,008 + PS417_25990 0.25 +0.2 5,616,008 + PS417_25990 0.25 +0.6 5,616,009 - PS417_25990 0.25 -0.4 5,616,009 - PS417_25990 0.25 -0.8 5,616,070 - PS417_25990 0.32 +0.1 5,616,657 + +0.4 5,617,300 - +0.0 5,617,327 - -0.2 5,617,327 - -0.4 5,617,394 - +0.3
Or see this region's nucleotide sequence