Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19685 and PS417_19690 are separated by 18 nucleotides PS417_19690 and PS417_19695 are separated by 155 nucleotides PS417_19695 and PS417_19700 overlap by 4 nucleotides PS417_19700 and PS417_19705 are separated by 106 nucleotides
PS417_19685: PS417_19685 - protein phosphatase, at 4,278,867 to 4,279,655
_19685
PS417_19690: PS417_19690 - histidine kinase, at 4,279,674 to 4,280,060
_19690
PS417_19695: PS417_19695 - flagellar biosynthesis sigma factor, at 4,280,216 to 4,280,956
_19695
PS417_19700: PS417_19700 - cobyrinic acid a,c-diamide synthase, at 4,280,953 to 4,281,786
_19700
PS417_19705: PS417_19705 - flagellar biosynthesis regulator FlhF, at 4,281,893 to 4,283,209
_19705
Position (kb) 4280
4281 Strain fitness (log2 ratio) -1
0
1 at 4279.384 kb on + strand, within PS417_19685 at 4279.384 kb on + strand, within PS417_19685 at 4279.385 kb on - strand, within PS417_19685 at 4279.385 kb on - strand, within PS417_19685 at 4279.385 kb on - strand, within PS417_19685 at 4279.385 kb on - strand, within PS417_19685 at 4279.385 kb on - strand, within PS417_19685 at 4279.385 kb on - strand, within PS417_19685 at 4279.647 kb on - strand at 4279.656 kb on - strand at 4279.747 kb on + strand, within PS417_19690 at 4279.848 kb on + strand, within PS417_19690 at 4279.848 kb on + strand, within PS417_19690 at 4279.848 kb on + strand, within PS417_19690 at 4279.848 kb on + strand, within PS417_19690 at 4279.849 kb on - strand, within PS417_19690 at 4280.080 kb on + strand at 4280.125 kb on + strand at 4280.126 kb on - strand at 4280.126 kb on - strand at 4280.347 kb on - strand, within PS417_19695 at 4280.446 kb on + strand, within PS417_19695 at 4280.446 kb on + strand, within PS417_19695 at 4280.533 kb on + strand, within PS417_19695 at 4280.554 kb on - strand, within PS417_19695 at 4280.736 kb on + strand, within PS417_19695 at 4280.736 kb on + strand, within PS417_19695 at 4280.737 kb on - strand at 4280.737 kb on - strand, within PS417_19695 at 4280.769 kb on + strand, within PS417_19695 at 4280.769 kb on + strand, within PS417_19695 at 4280.770 kb on - strand, within PS417_19695 at 4280.770 kb on - strand, within PS417_19695 at 4280.941 kb on - strand at 4280.956 kb on + strand at 4281.823 kb on - strand at 4281.864 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 4,279,384 + PS417_19685 0.66 +0.2 4,279,384 + PS417_19685 0.66 +0.1 4,279,385 - PS417_19685 0.66 +0.0 4,279,385 - PS417_19685 0.66 -0.1 4,279,385 - PS417_19685 0.66 -0.6 4,279,385 - PS417_19685 0.66 +0.3 4,279,385 - PS417_19685 0.66 +0.3 4,279,385 - PS417_19685 0.66 -0.3 4,279,647 - +0.1 4,279,656 - -0.1 4,279,747 + PS417_19690 0.19 +0.0 4,279,848 + PS417_19690 0.45 +1.0 4,279,848 + PS417_19690 0.45 +0.0 4,279,848 + PS417_19690 0.45 -0.1 4,279,848 + PS417_19690 0.45 +0.0 4,279,849 - PS417_19690 0.45 -0.6 4,280,080 + -0.0 4,280,125 + +1.0 4,280,126 - +0.0 4,280,126 - -0.3 4,280,347 - PS417_19695 0.18 +0.6 4,280,446 + PS417_19695 0.31 +0.0 4,280,446 + PS417_19695 0.31 +0.5 4,280,533 + PS417_19695 0.43 +0.2 4,280,554 - PS417_19695 0.46 +1.6 4,280,736 + PS417_19695 0.70 +0.3 4,280,736 + PS417_19695 0.70 +0.7 4,280,737 - +1.4 4,280,737 - PS417_19695 0.70 +0.3 4,280,769 + PS417_19695 0.75 +0.1 4,280,769 + PS417_19695 0.75 +0.4 4,280,770 - PS417_19695 0.75 -0.3 4,280,770 - PS417_19695 0.75 +0.3 4,280,941 - +0.7 4,280,956 + +0.1 4,281,823 - +1.1 4,281,864 - -0.7
Or see this region's nucleotide sequence