Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_18005 and PS417_18010 are separated by 96 nucleotides PS417_18010 and PS417_18015 are separated by 45 nucleotides PS417_18015 and PS417_18020 are separated by 10 nucleotides
PS417_18005: PS417_18005 - hypothetical protein, at 3,925,463 to 3,927,391
_18005
PS417_18010: PS417_18010 - acid phosphatase, at 3,927,488 to 3,928,312
_18010
PS417_18015: PS417_18015 - membrane protein, at 3,928,358 to 3,929,131
_18015
PS417_18020: PS417_18020 - hypothetical protein, at 3,929,142 to 3,929,534
_18020
Position (kb)
3927
3928
3929 Strain fitness (log2 ratio)
-1
0
1 at 3926.510 kb on - strand at 3926.587 kb on + strand, within PS417_18005 at 3926.588 kb on - strand, within PS417_18005 at 3926.630 kb on + strand, within PS417_18005 at 3926.676 kb on - strand, within PS417_18005 at 3926.676 kb on - strand at 3926.741 kb on + strand, within PS417_18005 at 3926.769 kb on + strand, within PS417_18005 at 3926.913 kb on + strand, within PS417_18005 at 3926.913 kb on + strand, within PS417_18005 at 3927.025 kb on + strand, within PS417_18005 at 3927.075 kb on + strand, within PS417_18005 at 3927.075 kb on + strand, within PS417_18005 at 3927.076 kb on - strand, within PS417_18005 at 3927.176 kb on + strand, within PS417_18005 at 3927.251 kb on + strand at 3928.167 kb on - strand, within PS417_18010 at 3928.214 kb on + strand, within PS417_18010 at 3928.227 kb on - strand, within PS417_18010 at 3928.563 kb on + strand, within PS417_18015 at 3928.564 kb on - strand, within PS417_18015 at 3929.151 kb on - strand at 3929.302 kb on + strand, within PS417_18020 at 3929.303 kb on - strand, within PS417_18020
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 3,926,510 - -1.4 3,926,587 + PS417_18005 0.58 +0.4 3,926,588 - PS417_18005 0.58 +0.1 3,926,630 + PS417_18005 0.60 -0.1 3,926,676 - PS417_18005 0.63 +0.0 3,926,676 - -1.5 3,926,741 + PS417_18005 0.66 -0.2 3,926,769 + PS417_18005 0.68 +0.1 3,926,913 + PS417_18005 0.75 +0.1 3,926,913 + PS417_18005 0.75 -0.5 3,927,025 + PS417_18005 0.81 +0.1 3,927,075 + PS417_18005 0.84 +0.5 3,927,075 + PS417_18005 0.84 +1.1 3,927,076 - PS417_18005 0.84 +0.1 3,927,176 + PS417_18005 0.89 -0.6 3,927,251 + +0.3 3,928,167 - PS417_18010 0.82 -0.2 3,928,214 + PS417_18010 0.88 -0.6 3,928,227 - PS417_18010 0.90 -0.5 3,928,563 + PS417_18015 0.26 +0.8 3,928,564 - PS417_18015 0.27 -1.3 3,929,151 - +0.2 3,929,302 + PS417_18020 0.41 +0.3 3,929,303 - PS417_18020 0.41 +0.2
Or see this region's nucleotide sequence