Strain Fitness in Pseudomonas simiae WCS417 around PS417_01525

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_01520 and PS417_01525 are separated by 87 nucleotidesPS417_01525 and PS417_01530 are separated by 119 nucleotidesPS417_01530 and PS417_01535 are separated by 141 nucleotidesPS417_01535 and PS417_01540 are separated by 103 nucleotides PS417_01520: PS417_01520 - arsenic resistance protein, at 336,753 to 337,718 _01520 PS417_01525: PS417_01525 - membrane protein, at 337,806 to 339,398 _01525 PS417_01530: PS417_01530 - hypothetical protein, at 339,518 to 340,075 _01530 PS417_01535: PS417_01535 - tRNA-Phe, at 340,217 to 340,292 _01535 PS417_01540: PS417_01540 - iron transporter, at 340,396 to 340,668 _01540 Position (kb) 337 338 339 340Strain fitness (log2 ratio) -1 0 1 2at 336.894 kb on + strand, within PS417_01520at 336.894 kb on + strand, within PS417_01520at 337.004 kb on - strand, within PS417_01520at 337.063 kb on + strand, within PS417_01520at 337.067 kb on - strand, within PS417_01520at 337.067 kb on - strand, within PS417_01520at 337.086 kb on + strand, within PS417_01520at 337.185 kb on + strand, within PS417_01520at 337.185 kb on + strand, within PS417_01520at 337.185 kb on + strand, within PS417_01520at 337.289 kb on - strand, within PS417_01520at 337.455 kb on + strand, within PS417_01520at 337.455 kb on + strand, within PS417_01520at 337.455 kb on + strand, within PS417_01520at 337.674 kb on + strandat 337.674 kb on + strandat 337.773 kb on + strandat 337.773 kb on + strandat 337.773 kb on + strandat 337.774 kb on - strandat 337.774 kb on - strandat 337.774 kb on - strandat 337.789 kb on - strandat 337.840 kb on + strandat 338.250 kb on + strand, within PS417_01525at 338.250 kb on + strand, within PS417_01525at 338.368 kb on - strand, within PS417_01525at 338.368 kb on - strand, within PS417_01525at 338.431 kb on + strand, within PS417_01525at 338.745 kb on + strand, within PS417_01525at 338.745 kb on + strand, within PS417_01525at 338.746 kb on - strand, within PS417_01525at 338.746 kb on - strand, within PS417_01525at 338.746 kb on - strand, within PS417_01525at 338.746 kb on - strand, within PS417_01525at 338.748 kb on + strand, within PS417_01525at 338.748 kb on + strand, within PS417_01525at 338.749 kb on - strand, within PS417_01525at 338.790 kb on + strand, within PS417_01525at 338.882 kb on - strand, within PS417_01525at 338.882 kb on - strand, within PS417_01525at 338.882 kb on - strand, within PS417_01525at 339.053 kb on - strand, within PS417_01525at 339.158 kb on + strand, within PS417_01525at 339.193 kb on - strand, within PS417_01525at 339.193 kb on - strand, within PS417_01525at 339.226 kb on + strand, within PS417_01525at 339.227 kb on - strand, within PS417_01525at 339.231 kb on - strand, within PS417_01525at 339.415 kb on + strandat 339.451 kb on - strandat 339.451 kb on - strandat 339.489 kb on + strandat 339.529 kb on - strandat 339.595 kb on + strand, within PS417_01530at 339.680 kb on + strand, within PS417_01530at 339.681 kb on - strand, within PS417_01530at 339.845 kb on - strand, within PS417_01530at 339.892 kb on + strand, within PS417_01530at 339.920 kb on + strand, within PS417_01530at 339.930 kb on - strand, within PS417_01530at 339.930 kb on - strand, within PS417_01530at 339.930 kb on - strand, within PS417_01530at 339.932 kb on + strand, within PS417_01530at 339.932 kb on + strand, within PS417_01530at 339.933 kb on - strand, within PS417_01530at 339.933 kb on - strand, within PS417_01530at 339.933 kb on - strand, within PS417_01530at 340.018 kb on - strand, within PS417_01530at 340.035 kb on + strandat 340.036 kb on - strandat 340.213 kb on - strandat 340.370 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
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336,894 + PS417_01520 0.15 -0.0
336,894 + PS417_01520 0.15 +0.3
337,004 - PS417_01520 0.26 -0.3
337,063 + PS417_01520 0.32 -0.5
337,067 - PS417_01520 0.33 +0.1
337,067 - PS417_01520 0.33 -0.3
337,086 + PS417_01520 0.34 +0.0
337,185 + PS417_01520 0.45 -0.0
337,185 + PS417_01520 0.45 -0.0
337,185 + PS417_01520 0.45 +0.0
337,289 - PS417_01520 0.55 +0.0
337,455 + PS417_01520 0.73 -1.0
337,455 + PS417_01520 0.73 -0.3
337,455 + PS417_01520 0.73 -0.5
337,674 + +0.4
337,674 + +0.2
337,773 + -0.1
337,773 + +0.3
337,773 + +0.0
337,774 - +0.3
337,774 - -0.2
337,774 - +1.3
337,789 - +0.2
337,840 + -0.4
338,250 + PS417_01525 0.28 -0.6
338,250 + PS417_01525 0.28 +0.0
338,368 - PS417_01525 0.35 -0.1
338,368 - PS417_01525 0.35 -0.2
338,431 + PS417_01525 0.39 -0.2
338,745 + PS417_01525 0.59 +0.6
338,745 + PS417_01525 0.59 -0.0
338,746 - PS417_01525 0.59 -0.3
338,746 - PS417_01525 0.59 +0.4
338,746 - PS417_01525 0.59 -1.1
338,746 - PS417_01525 0.59 +0.2
338,748 + PS417_01525 0.59 +0.2
338,748 + PS417_01525 0.59 +0.4
338,749 - PS417_01525 0.59 +0.4
338,790 + PS417_01525 0.62 +0.0
338,882 - PS417_01525 0.68 +0.6
338,882 - PS417_01525 0.68 -0.7
338,882 - PS417_01525 0.68 +0.7
339,053 - PS417_01525 0.78 +0.4
339,158 + PS417_01525 0.85 -0.3
339,193 - PS417_01525 0.87 -0.2
339,193 - PS417_01525 0.87 -0.0
339,226 + PS417_01525 0.89 +0.3
339,227 - PS417_01525 0.89 +0.3
339,231 - PS417_01525 0.89 -0.0
339,415 + -1.1
339,451 - +1.0
339,451 - +0.8
339,489 + -0.2
339,529 - +0.0
339,595 + PS417_01530 0.14 +2.0
339,680 + PS417_01530 0.29 -0.1
339,681 - PS417_01530 0.29 -0.1
339,845 - PS417_01530 0.59 +0.7
339,892 + PS417_01530 0.67 -0.2
339,920 + PS417_01530 0.72 +0.1
339,930 - PS417_01530 0.74 -0.2
339,930 - PS417_01530 0.74 -0.2
339,930 - PS417_01530 0.74 +0.2
339,932 + PS417_01530 0.74 -0.0
339,932 + PS417_01530 0.74 +0.0
339,933 - PS417_01530 0.74 +0.3
339,933 - PS417_01530 0.74 -0.1
339,933 - PS417_01530 0.74 +0.1
340,018 - PS417_01530 0.90 +0.3
340,035 + -0.1
340,036 - -0.8
340,213 - -0.7
340,370 - +0.6

Or see this region's nucleotide sequence