Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00080 and PS417_00085 are separated by 21 nucleotides PS417_00085 and PS417_00090 overlap by 4 nucleotides
PS417_00080: PS417_00080 - potassium transporter peripheral membrane component, at 17,327 to 18,700
_00080
PS417_00085: PS417_00085 - 16S rRNA methyltransferase, at 18,722 to 20,032
_00085
PS417_00090: PS417_00090 - methionyl-tRNA formyltransferase, at 20,029 to 20,982
_00090
Position (kb) 18
19
20
21 Strain fitness (log2 ratio) -1
0
1
2 at 17.767 kb on + strand, within PS417_00080 at 17.767 kb on + strand, within PS417_00080 at 17.768 kb on - strand, within PS417_00080 at 17.768 kb on - strand, within PS417_00080 at 17.768 kb on - strand, within PS417_00080 at 17.768 kb on - strand, within PS417_00080 at 17.768 kb on - strand, within PS417_00080 at 18.007 kb on + strand, within PS417_00080 at 18.009 kb on + strand, within PS417_00080 at 18.009 kb on + strand, within PS417_00080 at 18.010 kb on - strand, within PS417_00080 at 18.010 kb on - strand, within PS417_00080 at 18.222 kb on + strand, within PS417_00080 at 18.309 kb on + strand, within PS417_00080 at 18.309 kb on + strand, within PS417_00080 at 18.440 kb on - strand, within PS417_00080 at 18.440 kb on - strand, within PS417_00080 at 18.440 kb on - strand, within PS417_00080 at 18.557 kb on + strand, within PS417_00080 at 18.557 kb on + strand, within PS417_00080 at 18.557 kb on + strand, within PS417_00080 at 18.557 kb on + strand, within PS417_00080 at 18.558 kb on - strand, within PS417_00080 at 18.558 kb on - strand, within PS417_00080 at 18.763 kb on - strand at 18.924 kb on + strand, within PS417_00085 at 19.777 kb on - strand, within PS417_00085 at 19.777 kb on - strand, within PS417_00085 at 20.997 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 17,767 + PS417_00080 0.32 -0.7 17,767 + PS417_00080 0.32 -0.4 17,768 - PS417_00080 0.32 +0.4 17,768 - PS417_00080 0.32 -0.0 17,768 - PS417_00080 0.32 -0.5 17,768 - PS417_00080 0.32 -0.3 17,768 - PS417_00080 0.32 -0.5 18,007 + PS417_00080 0.49 +0.2 18,009 + PS417_00080 0.50 -1.6 18,009 + PS417_00080 0.50 -0.3 18,010 - PS417_00080 0.50 +0.5 18,010 - PS417_00080 0.50 -0.2 18,222 + PS417_00080 0.65 +0.3 18,309 + PS417_00080 0.71 -0.6 18,309 + PS417_00080 0.71 -0.6 18,440 - PS417_00080 0.81 -1.1 18,440 - PS417_00080 0.81 +1.9 18,440 - PS417_00080 0.81 +0.2 18,557 + PS417_00080 0.90 -0.5 18,557 + PS417_00080 0.90 +0.0 18,557 + PS417_00080 0.90 +0.1 18,557 + PS417_00080 0.90 -0.0 18,558 - PS417_00080 0.90 -0.6 18,558 - PS417_00080 0.90 -0.2 18,763 - +0.4 18,924 + PS417_00085 0.15 -0.3 19,777 - PS417_00085 0.80 -0.8 19,777 - PS417_00085 0.80 -0.9 20,997 - -0.2
Or see this region's nucleotide sequence