Strain Fitness in Pseudomonas simiae WCS417 around PS417_06250

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_06245 and PS417_06250 are separated by 74 nucleotidesPS417_06250 and PS417_06255 are separated by 45 nucleotidesPS417_06255 and PS417_06260 are separated by 3 nucleotides PS417_06245: PS417_06245 - zinc metallopeptidase RseP, at 1,384,901 to 1,386,253 _06245 PS417_06250: PS417_06250 - membrane protein, at 1,386,328 to 1,388,715 _06250 PS417_06255: PS417_06255 - Skp, at 1,388,761 to 1,389,264 _06255 PS417_06260: PS417_06260 - UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase, at 1,389,268 to 1,390,323 _06260 Position (kb) 1386 1387 1388 1389Strain fitness (log2 ratio) -2 -1 0 1at 1385.356 kb on + strand, within PS417_06245at 1385.544 kb on + strand, within PS417_06245at 1385.545 kb on - strand, within PS417_06245at 1386.219 kb on + strandat 1388.755 kb on + strandat 1388.766 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
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1,385,356 + PS417_06245 0.34 -0.8
1,385,544 + PS417_06245 0.48 -0.4
1,385,545 - PS417_06245 0.48 -2.3
1,386,219 + +0.3
1,388,755 + -0.8
1,388,766 + -1.4

Or see this region's nucleotide sequence