Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00370 and PS417_00375 are separated by 37 nucleotides PS417_00375 and PS417_00380 overlap by 8 nucleotides PS417_00380 and PS417_00385 overlap by 1 nucleotides PS417_00385 and PS417_00390 are separated by 67 nucleotides
PS417_00370: PS417_00370 - hypothetical protein, at 72,707 to 73,426
_00370
PS417_00375: PS417_00375 - membrane protein, at 73,464 to 74,252
_00375
PS417_00380: PS417_00380 - zinc ABC transporter ATP-binding protein, at 74,245 to 75,030
_00380
PS417_00385: PS417_00385 - Fur family transcriptional regulator, at 75,030 to 75,512
_00385
PS417_00390: PS417_00390 - ABC transporter substrate-binding protein, at 75,580 to 76,500
_00390
Position (kb) 74
75
76 Strain fitness (log2 ratio) -2
-1
0
1 at 73.440 kb on + strand at 73.465 kb on + strand at 73.887 kb on - strand, within PS417_00375 at 73.913 kb on - strand, within PS417_00375 at 74.155 kb on + strand, within PS417_00375 at 74.262 kb on + strand at 74.348 kb on + strand, within PS417_00380 at 74.550 kb on - strand at 74.550 kb on - strand, within PS417_00380 at 75.347 kb on - strand, within PS417_00385 at 75.543 kb on - strand at 75.545 kb on + strand at 75.546 kb on - strand at 75.660 kb on - strand at 75.756 kb on + strand, within PS417_00390 at 75.756 kb on + strand, within PS417_00390 at 75.757 kb on - strand, within PS417_00390 at 75.760 kb on + strand, within PS417_00390 at 75.760 kb on + strand, within PS417_00390 at 75.761 kb on - strand, within PS417_00390
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 73,440 + -0.0 73,465 + +0.1 73,887 - PS417_00375 0.54 -0.3 73,913 - PS417_00375 0.57 +0.2 74,155 + PS417_00375 0.88 -0.7 74,262 + -0.5 74,348 + PS417_00380 0.13 -0.8 74,550 - +1.1 74,550 - PS417_00380 0.39 -0.7 75,347 - PS417_00385 0.66 -0.8 75,543 - +0.4 75,545 + -0.2 75,546 - -0.3 75,660 - -1.8 75,756 + PS417_00390 0.19 -0.3 75,756 + PS417_00390 0.19 -0.7 75,757 - PS417_00390 0.19 -0.5 75,760 + PS417_00390 0.20 -0.6 75,760 + PS417_00390 0.20 -0.5 75,761 - PS417_00390 0.20 +0.4
Or see this region's nucleotide sequence