Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00340 and PS417_00345 overlap by 4 nucleotides PS417_00345 and PS417_00350 are separated by 161 nucleotides PS417_00350 and PS417_00355 are separated by 51 nucleotides PS417_00355 and PS417_00360 overlap by 1 nucleotides
PS417_00340: PS417_00340 - protoheme IX farnesyltransferase, at 66,005 to 66,904
_00340
PS417_00345: PS417_00345 - copper-binding protein, at 66,901 to 67,536
_00345
PS417_00350: PS417_00350 - methionine ABC transporter substrate-binding protein, at 67,698 to 68,468
_00350
PS417_00355: PS417_00355 - metal ABC transporter permease, at 68,520 to 69,194
_00355
PS417_00360: PS417_00360 - methionine ABC transporter ATP-binding protein, at 69,194 to 70,201
_00360
Position (kb) 67
68
69 Strain fitness (log2 ratio) -2
-1
0
1
2 at 66.707 kb on + strand, within PS417_00340 at 66.708 kb on - strand, within PS417_00340 at 66.708 kb on - strand, within PS417_00340 at 66.792 kb on - strand, within PS417_00340 at 66.833 kb on + strand at 66.833 kb on + strand at 66.838 kb on + strand at 66.839 kb on - strand at 66.839 kb on - strand at 66.845 kb on + strand at 66.888 kb on - strand at 66.900 kb on + strand at 66.900 kb on + strand at 66.900 kb on + strand at 66.900 kb on + strand at 67.225 kb on - strand, within PS417_00345 at 67.408 kb on + strand, within PS417_00345 at 67.409 kb on - strand, within PS417_00345 at 67.409 kb on - strand, within PS417_00345 at 67.409 kb on - strand, within PS417_00345 at 67.530 kb on + strand at 67.530 kb on + strand at 67.530 kb on + strand at 67.531 kb on - strand at 67.531 kb on - strand at 67.535 kb on - strand at 67.727 kb on - strand at 67.834 kb on + strand, within PS417_00350 at 67.835 kb on - strand, within PS417_00350 at 68.146 kb on + strand, within PS417_00350 at 68.146 kb on + strand, within PS417_00350 at 68.286 kb on + strand, within PS417_00350 at 68.286 kb on + strand, within PS417_00350 at 68.695 kb on + strand, within PS417_00355 at 68.696 kb on - strand, within PS417_00355 at 68.696 kb on - strand, within PS417_00355 at 68.709 kb on - strand, within PS417_00355 at 69.090 kb on + strand, within PS417_00355 at 69.196 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 66,707 + PS417_00340 0.78 +0.6 66,708 - PS417_00340 0.78 +0.1 66,708 - PS417_00340 0.78 -0.2 66,792 - PS417_00340 0.87 -0.0 66,833 + +2.1 66,833 + -0.6 66,838 + +0.6 66,839 - -0.0 66,839 - +0.6 66,845 + +0.1 66,888 - +0.2 66,900 + +0.4 66,900 + -0.2 66,900 + +0.5 66,900 + +0.1 67,225 - PS417_00345 0.51 +0.5 67,408 + PS417_00345 0.80 -1.2 67,409 - PS417_00345 0.80 +2.1 67,409 - PS417_00345 0.80 +1.5 67,409 - PS417_00345 0.80 +0.1 67,530 + +0.1 67,530 + -0.1 67,530 + +0.2 67,531 - -0.6 67,531 - -0.0 67,535 - +0.0 67,727 - -1.9 67,834 + PS417_00350 0.18 -1.5 67,835 - PS417_00350 0.18 -0.2 68,146 + PS417_00350 0.58 -0.6 68,146 + PS417_00350 0.58 -0.6 68,286 + PS417_00350 0.76 -0.2 68,286 + PS417_00350 0.76 -0.3 68,695 + PS417_00355 0.26 -0.9 68,696 - PS417_00355 0.26 -0.1 68,696 - PS417_00355 0.26 -0.4 68,709 - PS417_00355 0.28 -0.1 69,090 + PS417_00355 0.84 +0.5 69,196 - +0.1
Or see this region's nucleotide sequence