Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_28220 and PS417_28225 overlap by 23 nucleotides PS417_28225 and PS417_28230 are separated by 17 nucleotides PS417_28230 and PS417_28235 are separated by 161 nucleotides
PS417_28220: PS417_28220 - LysR family transcriptional regulator, at 6,093,366 to 6,094,340
_28220
PS417_28225: PS417_28225 - hypothetical protein, at 6,094,318 to 6,094,611
_28225
PS417_28230: PS417_28230 - transcriptional regulator, at 6,094,629 to 6,095,495
_28230
PS417_28235: PS417_28235 - diguanylate cyclase, at 6,095,657 to 6,098,530
_28235
Position (kb)
6094
6095
6096 Strain fitness (log2 ratio)
-1
0
1 at 6093.949 kb on - strand, within PS417_28220 at 6093.949 kb on - strand, within PS417_28220 at 6094.075 kb on + strand, within PS417_28220 at 6094.559 kb on + strand, within PS417_28225 at 6094.795 kb on + strand, within PS417_28230 at 6094.795 kb on + strand at 6094.796 kb on - strand, within PS417_28230 at 6094.796 kb on - strand, within PS417_28230 at 6095.095 kb on + strand, within PS417_28230 at 6095.096 kb on - strand, within PS417_28230 at 6095.215 kb on + strand, within PS417_28230 at 6095.216 kb on - strand, within PS417_28230 at 6095.477 kb on + strand at 6095.478 kb on - strand at 6095.533 kb on - strand at 6095.631 kb on + strand at 6095.631 kb on + strand at 6095.727 kb on + strand at 6095.728 kb on - strand at 6095.907 kb on + strand at 6095.931 kb on + strand at 6096.066 kb on + strand, within PS417_28235 at 6096.067 kb on - strand, within PS417_28235 at 6096.272 kb on - strand, within PS417_28235 at 6096.372 kb on + strand, within PS417_28235 at 6096.372 kb on + strand, within PS417_28235 at 6096.428 kb on + strand, within PS417_28235 at 6096.428 kb on + strand, within PS417_28235 at 6096.429 kb on - strand, within PS417_28235
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 6,093,949 - PS417_28220 0.60 -0.6 6,093,949 - PS417_28220 0.60 -0.4 6,094,075 + PS417_28220 0.73 -0.5 6,094,559 + PS417_28225 0.82 -0.2 6,094,795 + PS417_28230 0.19 -0.3 6,094,795 + +0.1 6,094,796 - PS417_28230 0.19 -0.3 6,094,796 - PS417_28230 0.19 -0.6 6,095,095 + PS417_28230 0.54 -0.7 6,095,096 - PS417_28230 0.54 -0.2 6,095,215 + PS417_28230 0.68 +0.4 6,095,216 - PS417_28230 0.68 -0.8 6,095,477 + -0.9 6,095,478 - -1.2 6,095,533 - +1.1 6,095,631 + -0.0 6,095,631 + +0.1 6,095,727 + +0.5 6,095,728 - +0.2 6,095,907 + -0.2 6,095,931 + +0.4 6,096,066 + PS417_28235 0.14 -0.2 6,096,067 - PS417_28235 0.14 +0.0 6,096,272 - PS417_28235 0.21 +0.0 6,096,372 + PS417_28235 0.25 +0.2 6,096,372 + PS417_28235 0.25 -0.5 6,096,428 + PS417_28235 0.27 -0.1 6,096,428 + PS417_28235 0.27 -0.1 6,096,429 - PS417_28235 0.27 +0.5
Or see this region's nucleotide sequence