Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_21145 and PS417_21150 are separated by 132 nucleotides PS417_21150 and PS417_21155 are separated by 107 nucleotides PS417_21155 and PS417_21160 overlap by 41 nucleotides
PS417_21145: PS417_21145 - oxidoreductase, at 4,601,260 to 4,602,006
_21145
PS417_21150: PS417_21150 - HAD family hydrolase, at 4,602,139 to 4,602,828
_21150
PS417_21155: PS417_21155 - hypothetical protein, at 4,602,936 to 4,603,319
_21155
PS417_21160: PS417_21160 - membrane protein, at 4,603,279 to 4,604,247
_21160
Position (kb) 4602
4603
4604 Strain fitness (log2 ratio) -1
0
1 at 4601.936 kb on - strand at 4601.959 kb on + strand at 4602.008 kb on - strand at 4602.077 kb on - strand at 4602.088 kb on + strand at 4602.089 kb on - strand at 4602.215 kb on + strand, within PS417_21150 at 4602.216 kb on - strand, within PS417_21150 at 4602.589 kb on + strand, within PS417_21150 at 4602.641 kb on + strand, within PS417_21150 at 4602.641 kb on + strand, within PS417_21150 at 4602.641 kb on + strand, within PS417_21150 at 4602.641 kb on + strand, within PS417_21150 at 4602.641 kb on + strand, within PS417_21150 at 4602.642 kb on - strand, within PS417_21150 at 4602.744 kb on - strand, within PS417_21150 at 4602.902 kb on + strand at 4602.903 kb on - strand at 4602.909 kb on + strand at 4602.909 kb on + strand at 4602.909 kb on + strand at 4602.910 kb on - strand at 4602.910 kb on - strand at 4602.910 kb on - strand at 4602.910 kb on - strand at 4602.910 kb on - strand at 4603.176 kb on + strand, within PS417_21155 at 4603.176 kb on + strand, within PS417_21155 at 4603.176 kb on + strand, within PS417_21155 at 4603.176 kb on + strand, within PS417_21155 at 4603.176 kb on + strand at 4603.176 kb on + strand, within PS417_21155 at 4603.177 kb on - strand, within PS417_21155 at 4603.177 kb on - strand, within PS417_21155 at 4603.177 kb on - strand, within PS417_21155 at 4603.177 kb on - strand, within PS417_21155 at 4603.194 kb on + strand, within PS417_21155 at 4603.267 kb on - strand, within PS417_21155 at 4603.267 kb on - strand, within PS417_21155 at 4603.267 kb on - strand, within PS417_21155 at 4603.317 kb on + strand at 4603.317 kb on + strand at 4603.318 kb on - strand at 4603.318 kb on - strand at 4603.318 kb on - strand at 4603.318 kb on - strand at 4603.318 kb on - strand at 4603.318 kb on - strand at 4603.318 kb on - strand at 4603.412 kb on + strand, within PS417_21160 at 4603.642 kb on + strand, within PS417_21160 at 4603.642 kb on + strand, within PS417_21160 at 4603.656 kb on - strand, within PS417_21160 at 4603.917 kb on - strand, within PS417_21160 at 4603.922 kb on + strand, within PS417_21160 at 4603.939 kb on + strand, within PS417_21160 at 4603.940 kb on - strand, within PS417_21160 at 4603.944 kb on + strand, within PS417_21160 at 4604.113 kb on - strand, within PS417_21160 at 4604.231 kb on - strand at 4604.299 kb on - strand at 4604.299 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 4,601,936 - -0.0 4,601,959 + -0.1 4,602,008 - -0.1 4,602,077 - -0.5 4,602,088 + +0.0 4,602,089 - -0.3 4,602,215 + PS417_21150 0.11 -1.0 4,602,216 - PS417_21150 0.11 +0.4 4,602,589 + PS417_21150 0.65 +0.2 4,602,641 + PS417_21150 0.73 -0.8 4,602,641 + PS417_21150 0.73 +0.9 4,602,641 + PS417_21150 0.73 -0.2 4,602,641 + PS417_21150 0.73 -0.5 4,602,641 + PS417_21150 0.73 -0.5 4,602,642 - PS417_21150 0.73 -0.1 4,602,744 - PS417_21150 0.88 +0.3 4,602,902 + -0.3 4,602,903 - -0.4 4,602,909 + -0.3 4,602,909 + +0.1 4,602,909 + +0.4 4,602,910 - +0.2 4,602,910 - -0.1 4,602,910 - -0.6 4,602,910 - -0.4 4,602,910 - -0.6 4,603,176 + PS417_21155 0.62 +0.5 4,603,176 + PS417_21155 0.62 -0.3 4,603,176 + PS417_21155 0.62 -0.6 4,603,176 + PS417_21155 0.62 -0.3 4,603,176 + +1.1 4,603,176 + PS417_21155 0.62 +0.2 4,603,177 - PS417_21155 0.63 +0.3 4,603,177 - PS417_21155 0.63 -0.3 4,603,177 - PS417_21155 0.63 -0.0 4,603,177 - PS417_21155 0.63 -0.4 4,603,194 + PS417_21155 0.67 -0.4 4,603,267 - PS417_21155 0.86 +0.1 4,603,267 - PS417_21155 0.86 +0.0 4,603,267 - PS417_21155 0.86 +0.0 4,603,317 + -0.3 4,603,317 + +0.4 4,603,318 - -0.1 4,603,318 - +0.1 4,603,318 - -0.1 4,603,318 - -0.0 4,603,318 - +0.2 4,603,318 - -0.0 4,603,318 - -0.2 4,603,412 + PS417_21160 0.14 -0.4 4,603,642 + PS417_21160 0.37 +0.4 4,603,642 + PS417_21160 0.37 -0.1 4,603,656 - PS417_21160 0.39 +0.0 4,603,917 - PS417_21160 0.66 -0.1 4,603,922 + PS417_21160 0.66 -0.5 4,603,939 + PS417_21160 0.68 +0.0 4,603,940 - PS417_21160 0.68 -0.5 4,603,944 + PS417_21160 0.69 -0.6 4,604,113 - PS417_21160 0.86 -0.5 4,604,231 - +1.4 4,604,299 - -0.3 4,604,299 - -0.2
Or see this region's nucleotide sequence