Strain Fitness in Pseudomonas simiae WCS417 around PS417_02055

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_02045 and PS417_02050 overlap by 4 nucleotidesPS417_02050 and PS417_02055 overlap by 4 nucleotidesPS417_02055 and PS417_02060 overlap by 4 nucleotides PS417_02045: PS417_02045 - 3-oxoacyl-ACP synthase, at 458,809 to 459,975 _02045 PS417_02050: PS417_02050 - hypothetical protein, at 459,972 to 460,454 _02050 PS417_02055: PS417_02055 - methyltransferase, at 460,451 to 461,185 _02055 PS417_02060: PS417_02060 - FAD-dependent oxidoreductase, at 461,182 to 462,429 _02060 Position (kb) 460 461 462Strain fitness (log2 ratio) -1 0 1at 459.599 kb on + strand, within PS417_02045at 459.707 kb on + strand, within PS417_02045at 459.708 kb on - strand, within PS417_02045at 460.716 kb on + strand, within PS417_02055at 460.716 kb on + strand, within PS417_02055at 460.717 kb on - strand, within PS417_02055at 460.717 kb on - strand, within PS417_02055at 460.746 kb on + strand, within PS417_02055at 460.747 kb on - strand, within PS417_02055at 461.059 kb on + strand, within PS417_02055at 461.068 kb on + strand, within PS417_02055at 461.459 kb on - strand, within PS417_02060at 461.814 kb on + strand, within PS417_02060at 461.815 kb on - strand, within PS417_02060at 461.992 kb on + strand, within PS417_02060

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
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459,599 + PS417_02045 0.68 -0.3
459,707 + PS417_02045 0.77 -0.0
459,708 - PS417_02045 0.77 +0.4
460,716 + PS417_02055 0.36 -0.7
460,716 + PS417_02055 0.36 -0.2
460,717 - PS417_02055 0.36 +0.3
460,717 - PS417_02055 0.36 -0.4
460,746 + PS417_02055 0.40 -1.3
460,747 - PS417_02055 0.40 -0.0
461,059 + PS417_02055 0.83 -0.1
461,068 + PS417_02055 0.84 -0.3
461,459 - PS417_02060 0.22 -0.7
461,814 + PS417_02060 0.51 +0.0
461,815 - PS417_02060 0.51 -0.2
461,992 + PS417_02060 0.65 -0.2

Or see this region's nucleotide sequence