Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12130 and PS417_12135 are separated by 63 nucleotides PS417_12135 and PS417_12140 are separated by 43 nucleotides PS417_12140 and PS417_12145 are separated by 135 nucleotides
PS417_12130: PS417_12130 - ketodeoxygluconokinase, at 2,631,877 to 2,632,806
_12130
PS417_12135: PS417_12135 - peptidylprolyl isomerase, at 2,632,870 to 2,633,145
_12135
PS417_12140: PS417_12140 - histidine kinase, at 2,633,189 to 2,635,684
_12140
PS417_12145: PS417_12145 - acetoacetyl-CoA synthetase, at 2,635,820 to 2,637,778
_12145
Position (kb)
2633
2634
2635
2636 Strain fitness (log2 ratio)
-1
0
1 at 2632.279 kb on + strand, within PS417_12130 at 2632.280 kb on - strand, within PS417_12130 at 2632.435 kb on + strand, within PS417_12130 at 2632.786 kb on + strand at 2633.077 kb on + strand, within PS417_12135 at 2633.178 kb on + strand at 2633.178 kb on + strand at 2633.397 kb on - strand at 2633.710 kb on - strand, within PS417_12140 at 2633.835 kb on + strand, within PS417_12140 at 2633.835 kb on + strand, within PS417_12140 at 2634.504 kb on + strand, within PS417_12140 at 2634.504 kb on + strand, within PS417_12140 at 2634.505 kb on - strand, within PS417_12140 at 2634.547 kb on - strand, within PS417_12140 at 2634.654 kb on + strand, within PS417_12140 at 2634.654 kb on + strand, within PS417_12140 at 2634.654 kb on + strand, within PS417_12140 at 2634.655 kb on - strand, within PS417_12140 at 2634.693 kb on + strand, within PS417_12140 at 2634.696 kb on - strand, within PS417_12140 at 2634.696 kb on - strand, within PS417_12140 at 2634.696 kb on - strand, within PS417_12140 at 2635.060 kb on - strand, within PS417_12140 at 2635.302 kb on + strand, within PS417_12140 at 2635.376 kb on + strand, within PS417_12140 at 2635.506 kb on + strand at 2635.768 kb on + strand at 2635.801 kb on - strand at 2636.006 kb on - strand at 2636.301 kb on + strand, within PS417_12145 at 2636.565 kb on + strand, within PS417_12145
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 2,632,279 + PS417_12130 0.43 -0.3 2,632,280 - PS417_12130 0.43 -0.2 2,632,435 + PS417_12130 0.60 +1.3 2,632,786 + -0.0 2,633,077 + PS417_12135 0.75 -0.2 2,633,178 + -0.2 2,633,178 + +0.0 2,633,397 - -0.2 2,633,710 - PS417_12140 0.21 -0.2 2,633,835 + PS417_12140 0.26 -0.6 2,633,835 + PS417_12140 0.26 -1.2 2,634,504 + PS417_12140 0.53 -0.9 2,634,504 + PS417_12140 0.53 -0.3 2,634,505 - PS417_12140 0.53 -0.3 2,634,547 - PS417_12140 0.54 -1.7 2,634,654 + PS417_12140 0.59 -0.5 2,634,654 + PS417_12140 0.59 -0.7 2,634,654 + PS417_12140 0.59 -0.5 2,634,655 - PS417_12140 0.59 -0.1 2,634,693 + PS417_12140 0.60 +0.7 2,634,696 - PS417_12140 0.60 -1.1 2,634,696 - PS417_12140 0.60 +0.2 2,634,696 - PS417_12140 0.60 -0.2 2,635,060 - PS417_12140 0.75 -1.6 2,635,302 + PS417_12140 0.85 +0.5 2,635,376 + PS417_12140 0.88 +0.5 2,635,506 + -0.7 2,635,768 + -0.4 2,635,801 - +0.4 2,636,006 - -0.2 2,636,301 + PS417_12145 0.25 -1.2 2,636,565 + PS417_12145 0.38 -0.7
Or see this region's nucleotide sequence