Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11175 and PS417_11180 are separated by 173 nucleotides PS417_11180 and PS417_11185 overlap by 4 nucleotides PS417_11185 and PS417_11190 are separated by 79 nucleotides
PS417_11175: PS417_11175 - AraC family transcriptional regulator, at 2,397,187 to 2,398,125
_11175
PS417_11180: PS417_11180 - TetR family transcriptional regulator, at 2,398,299 to 2,398,928
_11180
PS417_11185: PS417_11185 - dimethylallyltransferase, at 2,398,925 to 2,399,602
_11185
PS417_11190: PS417_11190 - oxidoreductase, at 2,399,682 to 2,400,806
_11190
Position (kb)
2398
2399 Strain fitness (log2 ratio)
-1
0
1 at 2397.502 kb on + strand, within PS417_11175 at 2397.502 kb on + strand, within PS417_11175 at 2398.281 kb on - strand at 2398.397 kb on - strand, within PS417_11180 at 2398.432 kb on - strand, within PS417_11180 at 2398.437 kb on + strand, within PS417_11180 at 2398.437 kb on + strand, within PS417_11180 at 2398.438 kb on - strand, within PS417_11180 at 2398.495 kb on - strand, within PS417_11180 at 2398.908 kb on + strand at 2398.933 kb on - strand at 2398.936 kb on - strand at 2398.941 kb on + strand at 2398.942 kb on - strand at 2399.016 kb on - strand, within PS417_11185 at 2399.219 kb on + strand, within PS417_11185 at 2399.439 kb on + strand, within PS417_11185 at 2399.440 kb on - strand, within PS417_11185 at 2399.605 kb on + strand at 2399.605 kb on + strand at 2399.606 kb on - strand at 2399.637 kb on + strand at 2399.726 kb on + strand at 2399.741 kb on + strand at 2399.741 kb on + strand at 2399.814 kb on + strand, within PS417_11190 at 2399.887 kb on - strand, within PS417_11190 at 2399.908 kb on - strand, within PS417_11190
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 2,397,502 + PS417_11175 0.34 -0.4 2,397,502 + PS417_11175 0.34 -0.4 2,398,281 - -0.5 2,398,397 - PS417_11180 0.16 -0.6 2,398,432 - PS417_11180 0.21 -0.7 2,398,437 + PS417_11180 0.22 -0.1 2,398,437 + PS417_11180 0.22 +0.0 2,398,438 - PS417_11180 0.22 +0.2 2,398,495 - PS417_11180 0.31 -0.6 2,398,908 + -1.3 2,398,933 - +0.2 2,398,936 - -0.1 2,398,941 + -0.3 2,398,942 - +1.0 2,399,016 - PS417_11185 0.13 +0.1 2,399,219 + PS417_11185 0.43 +0.5 2,399,439 + PS417_11185 0.76 -0.2 2,399,440 - PS417_11185 0.76 -0.0 2,399,605 + +0.3 2,399,605 + -0.3 2,399,606 - +0.1 2,399,637 + +0.8 2,399,726 + -0.5 2,399,741 + +0.5 2,399,741 + -0.2 2,399,814 + PS417_11190 0.12 +0.1 2,399,887 - PS417_11190 0.18 -0.0 2,399,908 - PS417_11190 0.20 -0.1
Or see this region's nucleotide sequence