Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06450 and PS417_06455 are separated by 28 nucleotides PS417_06455 and PS417_06460 are separated by 3 nucleotides PS417_06460 and PS417_06465 are separated by 198 nucleotides PS417_06465 and PS417_06470 overlap by 4 nucleotides
PS417_06450: PS417_06450 - iron ABC transporter substrate-binding protein, at 1,430,511 to 1,431,626
_06450
PS417_06455: PS417_06455 - Fe(II)-dependent oxygenase, at 1,431,655 to 1,432,335
_06455
PS417_06460: PS417_06460 - TonB-dependent receptor, at 1,432,339 to 1,434,720
_06460
PS417_06465: PS417_06465 - transcriptional regulator, at 1,434,919 to 1,435,629
_06465
PS417_06470: PS417_06470 - histidine kinase, at 1,435,626 to 1,437,011
_06470
Position (kb)
1432
1433
1434
1435 Strain fitness (log2 ratio)
-1
0
1 at 1431.365 kb on - strand, within PS417_06450 at 1431.374 kb on - strand, within PS417_06450 at 1431.374 kb on - strand, within PS417_06450 at 1431.374 kb on - strand, within PS417_06450 at 1431.374 kb on - strand, within PS417_06450 at 1431.495 kb on + strand, within PS417_06450 at 1431.496 kb on - strand, within PS417_06450 at 1431.770 kb on - strand, within PS417_06455 at 1431.957 kb on - strand, within PS417_06455 at 1431.957 kb on - strand, within PS417_06455 at 1432.097 kb on + strand, within PS417_06455 at 1432.125 kb on + strand, within PS417_06455 at 1432.349 kb on + strand at 1432.349 kb on + strand at 1432.433 kb on + strand at 1432.434 kb on - strand at 1432.436 kb on + strand at 1432.436 kb on + strand at 1432.437 kb on - strand at 1432.553 kb on + strand at 1432.553 kb on + strand at 1432.587 kb on - strand, within PS417_06460 at 1432.616 kb on + strand, within PS417_06460 at 1432.616 kb on + strand, within PS417_06460 at 1432.617 kb on - strand, within PS417_06460 at 1432.632 kb on - strand, within PS417_06460 at 1432.650 kb on - strand, within PS417_06460 at 1432.691 kb on + strand, within PS417_06460 at 1432.691 kb on + strand at 1432.793 kb on + strand, within PS417_06460 at 1432.793 kb on + strand, within PS417_06460 at 1432.794 kb on - strand, within PS417_06460 at 1433.087 kb on + strand, within PS417_06460 at 1433.096 kb on + strand, within PS417_06460 at 1433.096 kb on + strand, within PS417_06460 at 1433.097 kb on - strand at 1433.097 kb on - strand, within PS417_06460 at 1433.324 kb on + strand, within PS417_06460 at 1433.324 kb on + strand, within PS417_06460 at 1433.324 kb on + strand, within PS417_06460 at 1433.324 kb on + strand, within PS417_06460 at 1433.324 kb on + strand, within PS417_06460 at 1433.423 kb on + strand, within PS417_06460 at 1433.424 kb on - strand, within PS417_06460 at 1433.627 kb on - strand, within PS417_06460 at 1433.627 kb on - strand, within PS417_06460 at 1434.050 kb on + strand, within PS417_06460 at 1434.051 kb on - strand, within PS417_06460 at 1434.069 kb on + strand, within PS417_06460 at 1434.069 kb on + strand, within PS417_06460 at 1434.070 kb on - strand, within PS417_06460 at 1434.070 kb on - strand at 1434.073 kb on + strand, within PS417_06460 at 1434.074 kb on - strand, within PS417_06460 at 1434.400 kb on - strand, within PS417_06460 at 1434.535 kb on + strand at 1434.535 kb on + strand at 1434.743 kb on - strand at 1434.772 kb on + strand at 1434.772 kb on + strand at 1434.772 kb on + strand at 1434.773 kb on - strand at 1434.773 kb on - strand at 1434.824 kb on + strand at 1434.835 kb on - strand at 1434.848 kb on - strand at 1435.219 kb on + strand, within PS417_06465 at 1435.220 kb on - strand, within PS417_06465 at 1435.370 kb on + strand, within PS417_06465 at 1435.370 kb on + strand, within PS417_06465
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 1,431,365 - PS417_06450 0.77 -0.2 1,431,374 - PS417_06450 0.77 -0.3 1,431,374 - PS417_06450 0.77 -0.1 1,431,374 - PS417_06450 0.77 +0.1 1,431,374 - PS417_06450 0.77 -0.5 1,431,495 + PS417_06450 0.88 -0.1 1,431,496 - PS417_06450 0.88 -0.1 1,431,770 - PS417_06455 0.17 +0.1 1,431,957 - PS417_06455 0.44 -0.5 1,431,957 - PS417_06455 0.44 -0.2 1,432,097 + PS417_06455 0.65 +0.2 1,432,125 + PS417_06455 0.69 -0.4 1,432,349 + +0.1 1,432,349 + -0.3 1,432,433 + -0.0 1,432,434 - +0.2 1,432,436 + -0.3 1,432,436 + +0.3 1,432,437 - -0.4 1,432,553 + +0.5 1,432,553 + +0.4 1,432,587 - PS417_06460 0.10 -0.3 1,432,616 + PS417_06460 0.12 +0.0 1,432,616 + PS417_06460 0.12 -0.5 1,432,617 - PS417_06460 0.12 +0.3 1,432,632 - PS417_06460 0.12 -0.8 1,432,650 - PS417_06460 0.13 +0.1 1,432,691 + PS417_06460 0.15 +0.1 1,432,691 + +1.5 1,432,793 + PS417_06460 0.19 +0.8 1,432,793 + PS417_06460 0.19 +0.5 1,432,794 - PS417_06460 0.19 -0.3 1,433,087 + PS417_06460 0.31 +0.1 1,433,096 + PS417_06460 0.32 -0.2 1,433,096 + PS417_06460 0.32 -0.0 1,433,097 - -0.3 1,433,097 - PS417_06460 0.32 -0.1 1,433,324 + PS417_06460 0.41 -0.1 1,433,324 + PS417_06460 0.41 +0.4 1,433,324 + PS417_06460 0.41 -0.0 1,433,324 + PS417_06460 0.41 +1.4 1,433,324 + PS417_06460 0.41 -0.3 1,433,423 + PS417_06460 0.46 +0.2 1,433,424 - PS417_06460 0.46 +0.1 1,433,627 - PS417_06460 0.54 +0.6 1,433,627 - PS417_06460 0.54 +0.9 1,434,050 + PS417_06460 0.72 -0.9 1,434,051 - PS417_06460 0.72 -0.1 1,434,069 + PS417_06460 0.73 -0.0 1,434,069 + PS417_06460 0.73 -0.2 1,434,070 - PS417_06460 0.73 +0.7 1,434,070 - +1.0 1,434,073 + PS417_06460 0.73 +0.3 1,434,074 - PS417_06460 0.73 -0.2 1,434,400 - PS417_06460 0.87 +0.2 1,434,535 + -0.3 1,434,535 + +0.0 1,434,743 - +0.5 1,434,772 + -0.1 1,434,772 + +0.0 1,434,772 + -0.2 1,434,773 - -0.7 1,434,773 - +1.0 1,434,824 + -0.7 1,434,835 - -0.0 1,434,848 - -0.2 1,435,219 + PS417_06465 0.42 -0.4 1,435,220 - PS417_06465 0.42 -0.0 1,435,370 + PS417_06465 0.63 -0.1 1,435,370 + PS417_06465 0.63 +0.0
Or see this region's nucleotide sequence