Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05725 and PS417_05730 overlap by 4 nucleotides PS417_05730 and PS417_05735 overlap by 4 nucleotides PS417_05735 and PS417_05740 are separated by 48 nucleotides PS417_05740 and PS417_05745 are separated by 1 nucleotides
PS417_05725: PS417_05725 - baseplate J protein, at 1,283,863 to 1,284,858
_05725
PS417_05730: PS417_05730 - tail protein, at 1,284,855 to 1,285,493
_05730
PS417_05735: PS417_05735 - tail fiber protein, at 1,285,490 to 1,286,020
_05735
PS417_05740: PS417_05740 - hypothetical protein, at 1,286,069 to 1,286,851
_05740
PS417_05745: PS417_05745 - hypothetical protein, at 1,286,853 to 1,287,155
_05745
Position (kb)
1285
1286
1287 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1284.492 kb on + strand, within PS417_05725 at 1284.599 kb on - strand, within PS417_05725 at 1284.683 kb on - strand, within PS417_05725 at 1284.849 kb on + strand at 1284.854 kb on + strand at 1284.854 kb on + strand at 1284.891 kb on - strand at 1284.891 kb on - strand at 1285.005 kb on + strand, within PS417_05730 at 1285.006 kb on - strand, within PS417_05730 at 1285.006 kb on - strand, within PS417_05730 at 1285.201 kb on - strand, within PS417_05730 at 1285.271 kb on + strand, within PS417_05730 at 1285.272 kb on - strand, within PS417_05730 at 1285.431 kb on - strand at 1285.455 kb on + strand at 1285.456 kb on - strand at 1285.456 kb on - strand at 1285.456 kb on - strand at 1285.757 kb on + strand at 1285.787 kb on + strand, within PS417_05735 at 1285.814 kb on + strand, within PS417_05735 at 1285.815 kb on - strand, within PS417_05735 at 1285.815 kb on - strand, within PS417_05735 at 1285.817 kb on - strand, within PS417_05735 at 1285.994 kb on - strand at 1286.000 kb on - strand at 1286.022 kb on - strand at 1286.114 kb on - strand at 1286.206 kb on - strand, within PS417_05740 at 1286.212 kb on + strand, within PS417_05740 at 1286.213 kb on - strand, within PS417_05740 at 1286.238 kb on - strand, within PS417_05740 at 1286.265 kb on + strand, within PS417_05740 at 1286.270 kb on - strand, within PS417_05740 at 1286.270 kb on - strand, within PS417_05740 at 1286.335 kb on + strand, within PS417_05740 at 1286.336 kb on - strand, within PS417_05740 at 1286.521 kb on + strand, within PS417_05740 at 1286.532 kb on - strand at 1286.532 kb on - strand, within PS417_05740 at 1286.532 kb on - strand, within PS417_05740 at 1286.532 kb on - strand, within PS417_05740 at 1286.618 kb on + strand, within PS417_05740 at 1286.619 kb on - strand, within PS417_05740 at 1286.619 kb on - strand, within PS417_05740 at 1286.635 kb on + strand at 1286.636 kb on - strand, within PS417_05740 at 1286.639 kb on + strand, within PS417_05740 at 1286.639 kb on + strand, within PS417_05740 at 1286.640 kb on - strand, within PS417_05740 at 1286.640 kb on - strand, within PS417_05740 at 1286.640 kb on - strand, within PS417_05740 at 1286.640 kb on - strand, within PS417_05740 at 1286.641 kb on + strand at 1286.641 kb on + strand, within PS417_05740 at 1286.808 kb on - strand at 1286.852 kb on + strand at 1286.875 kb on + strand at 1286.960 kb on - strand, within PS417_05745 at 1286.988 kb on - strand, within PS417_05745 at 1287.008 kb on + strand, within PS417_05745 at 1287.009 kb on - strand, within PS417_05745
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 1,284,492 + PS417_05725 0.63 +0.5 1,284,599 - PS417_05725 0.74 +0.9 1,284,683 - PS417_05725 0.82 -0.5 1,284,849 + +0.4 1,284,854 + -0.5 1,284,854 + +0.2 1,284,891 - +0.1 1,284,891 - +0.0 1,285,005 + PS417_05730 0.23 +0.0 1,285,006 - PS417_05730 0.24 -0.1 1,285,006 - PS417_05730 0.24 +0.1 1,285,201 - PS417_05730 0.54 +0.2 1,285,271 + PS417_05730 0.65 -0.1 1,285,272 - PS417_05730 0.65 -0.6 1,285,431 - -0.6 1,285,455 + -0.1 1,285,456 - -0.3 1,285,456 - +0.3 1,285,456 - +0.1 1,285,757 + +1.8 1,285,787 + PS417_05735 0.56 -0.6 1,285,814 + PS417_05735 0.61 -0.2 1,285,815 - PS417_05735 0.61 +0.3 1,285,815 - PS417_05735 0.61 -0.5 1,285,817 - PS417_05735 0.62 -1.9 1,285,994 - +0.3 1,286,000 - +0.6 1,286,022 - -0.6 1,286,114 - +0.3 1,286,206 - PS417_05740 0.17 -0.2 1,286,212 + PS417_05740 0.18 -0.3 1,286,213 - PS417_05740 0.18 -0.1 1,286,238 - PS417_05740 0.22 +0.3 1,286,265 + PS417_05740 0.25 +0.0 1,286,270 - PS417_05740 0.26 +0.1 1,286,270 - PS417_05740 0.26 +0.4 1,286,335 + PS417_05740 0.34 -0.5 1,286,336 - PS417_05740 0.34 -0.1 1,286,521 + PS417_05740 0.58 -0.6 1,286,532 - +0.7 1,286,532 - PS417_05740 0.59 -0.1 1,286,532 - PS417_05740 0.59 -0.0 1,286,532 - PS417_05740 0.59 +0.8 1,286,618 + PS417_05740 0.70 +1.6 1,286,619 - PS417_05740 0.70 +0.1 1,286,619 - PS417_05740 0.70 +0.2 1,286,635 + -1.6 1,286,636 - PS417_05740 0.72 +0.1 1,286,639 + PS417_05740 0.73 -0.3 1,286,639 + PS417_05740 0.73 -0.7 1,286,640 - PS417_05740 0.73 +0.5 1,286,640 - PS417_05740 0.73 -0.1 1,286,640 - PS417_05740 0.73 -0.0 1,286,640 - PS417_05740 0.73 +0.4 1,286,641 + -0.3 1,286,641 + PS417_05740 0.73 -0.4 1,286,808 - -0.4 1,286,852 + +0.0 1,286,875 + +0.3 1,286,960 - PS417_05745 0.35 -0.2 1,286,988 - PS417_05745 0.45 -0.2 1,287,008 + PS417_05745 0.51 -0.3 1,287,009 - PS417_05745 0.51 +0.1
Or see this region's nucleotide sequence