Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00450 and PS417_00455 are separated by 376 nucleotides PS417_00455 and PS417_00460 are separated by 79 nucleotides PS417_00460 and PS417_00465 are separated by 2 nucleotides PS417_00465 and PS417_00470 are separated by 728 nucleotides
PS417_00450: PS417_00450 - chemotaxis protein CheY, at 89,226 to 90,572
_00450
PS417_00455: PS417_00455 - hypothetical protein, at 90,949 to 91,233
_00455
PS417_00460: PS417_00460 - membrane protein, at 91,313 to 91,477
_00460
PS417_00465: PS417_00465 - hypothetical protein, at 91,480 to 91,947
_00465
PS417_00470: PS417_00470 - glutamate:protein symporter, at 92,676 to 94,007
_00470
Position (kb)
91
92 Strain fitness (log2 ratio)
-2
-1
0
1 at 90.535 kb on - strand at 90.589 kb on - strand at 90.594 kb on - strand at 90.594 kb on - strand at 90.610 kb on - strand at 90.643 kb on - strand at 90.675 kb on + strand at 90.675 kb on + strand at 90.676 kb on - strand at 90.676 kb on - strand at 90.676 kb on - strand at 90.692 kb on - strand at 90.729 kb on + strand at 90.730 kb on - strand at 90.908 kb on + strand at 90.908 kb on + strand at 90.909 kb on - strand at 90.925 kb on - strand at 90.981 kb on - strand, within PS417_00455 at 91.143 kb on - strand, within PS417_00455 at 91.143 kb on - strand, within PS417_00455 at 91.231 kb on + strand at 91.232 kb on - strand at 91.274 kb on - strand at 91.283 kb on - strand at 91.289 kb on + strand at 91.289 kb on + strand at 91.300 kb on - strand at 91.714 kb on + strand at 91.714 kb on + strand, within PS417_00465 at 91.714 kb on + strand at 91.715 kb on - strand, within PS417_00465 at 91.715 kb on - strand, within PS417_00465 at 91.801 kb on + strand, within PS417_00465 at 91.801 kb on + strand, within PS417_00465 at 91.802 kb on - strand, within PS417_00465 at 91.843 kb on - strand, within PS417_00465 at 91.935 kb on + strand at 91.936 kb on - strand at 91.936 kb on - strand at 91.942 kb on + strand at 91.942 kb on + strand at 91.945 kb on + strand at 91.945 kb on + strand at 91.945 kb on + strand at 91.945 kb on + strand at 91.945 kb on + strand at 91.946 kb on - strand at 91.946 kb on - strand at 91.946 kb on - strand at 91.946 kb on - strand at 91.946 kb on - strand at 92.090 kb on + strand at 92.090 kb on + strand at 92.185 kb on + strand at 92.207 kb on + strand at 92.207 kb on + strand at 92.207 kb on + strand at 92.207 kb on + strand at 92.208 kb on - strand at 92.208 kb on - strand at 92.254 kb on + strand at 92.254 kb on + strand at 92.258 kb on + strand at 92.259 kb on - strand at 92.279 kb on + strand at 92.279 kb on + strand at 92.280 kb on - strand at 92.531 kb on + strand at 92.531 kb on + strand at 92.557 kb on + strand at 92.648 kb on + strand at 92.663 kb on + strand at 92.663 kb on + strand at 92.663 kb on + strand at 92.669 kb on + strand at 92.693 kb on - strand at 92.813 kb on + strand, within PS417_00470 at 92.814 kb on - strand, within PS417_00470 at 92.814 kb on - strand, within PS417_00470 at 92.935 kb on - strand, within PS417_00470
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 90,535 - -0.8 90,589 - -0.0 90,594 - +0.3 90,594 - +0.3 90,610 - +1.7 90,643 - -0.0 90,675 + +1.6 90,675 + +0.1 90,676 - -0.0 90,676 - +1.0 90,676 - -0.2 90,692 - -0.8 90,729 + +0.7 90,730 - +0.1 90,908 + -2.7 90,908 + +0.1 90,909 - -0.2 90,925 - -0.4 90,981 - PS417_00455 0.11 -0.2 91,143 - PS417_00455 0.68 +0.2 91,143 - PS417_00455 0.68 -0.2 91,231 + -0.5 91,232 - +1.6 91,274 - +0.8 91,283 - +0.1 91,289 + -0.1 91,289 + +0.2 91,300 - -0.1 91,714 + +1.1 91,714 + PS417_00465 0.50 -0.3 91,714 + -0.7 91,715 - PS417_00465 0.50 -0.3 91,715 - PS417_00465 0.50 +0.2 91,801 + PS417_00465 0.69 +0.1 91,801 + PS417_00465 0.69 +0.5 91,802 - PS417_00465 0.69 +0.1 91,843 - PS417_00465 0.78 -0.4 91,935 + +0.3 91,936 - +0.4 91,936 - +0.2 91,942 + -0.5 91,942 + +0.2 91,945 + -0.4 91,945 + -0.3 91,945 + -0.5 91,945 + -0.9 91,945 + -0.4 91,946 - +0.2 91,946 - +0.1 91,946 - -0.7 91,946 - +0.6 91,946 - +0.0 92,090 + +0.6 92,090 + +0.2 92,185 + -0.7 92,207 + +0.0 92,207 + -0.3 92,207 + -0.1 92,207 + +0.2 92,208 - -0.3 92,208 - +0.5 92,254 + +0.3 92,254 + -0.1 92,258 + -0.3 92,259 - -0.9 92,279 + +0.1 92,279 + +0.1 92,280 - -0.2 92,531 + -0.1 92,531 + -0.2 92,557 + +1.5 92,648 + -0.2 92,663 + +0.7 92,663 + +0.7 92,663 + -1.5 92,669 + +0.9 92,693 - -0.4 92,813 + PS417_00470 0.10 +0.0 92,814 - PS417_00470 0.10 +0.4 92,814 - PS417_00470 0.10 -0.3 92,935 - PS417_00470 0.19 -1.0
Or see this region's nucleotide sequence