Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01885 and PS417_01890 are separated by 78 nucleotides PS417_01890 and PS417_01895 are separated by 27 nucleotides
PS417_01885: PS417_01885 - poly(3-hydroxyalkanoate) granule-associated protein PhaF, at 416,743 to 417,726
_01885
PS417_01890: PS417_01890 - TetR family transcriptional regulator, at 417,805 to 418,425
_01890
PS417_01895: PS417_01895 - poly(R)-hydroxyalkanoic acid synthase, at 418,453 to 420,135
_01895
Position (kb) 417
418
419 Strain fitness (log2 ratio) -1
0
1
2 at 416.869 kb on + strand, within PS417_01885 at 416.870 kb on - strand, within PS417_01885 at 416.870 kb on - strand, within PS417_01885 at 417.132 kb on + strand, within PS417_01885 at 417.621 kb on + strand, within PS417_01885 at 417.622 kb on - strand, within PS417_01885 at 417.806 kb on + strand at 417.807 kb on - strand at 417.824 kb on + strand at 417.825 kb on - strand at 417.868 kb on + strand, within PS417_01890 at 417.868 kb on + strand, within PS417_01890 at 417.868 kb on + strand, within PS417_01890 at 417.899 kb on + strand, within PS417_01890 at 417.900 kb on - strand, within PS417_01890 at 417.914 kb on - strand, within PS417_01890 at 418.152 kb on - strand, within PS417_01890 at 418.193 kb on + strand, within PS417_01890 at 418.194 kb on - strand, within PS417_01890 at 418.194 kb on - strand, within PS417_01890 at 418.277 kb on - strand, within PS417_01890 at 418.304 kb on + strand, within PS417_01890 at 418.304 kb on + strand, within PS417_01890 at 418.307 kb on + strand, within PS417_01890 at 418.436 kb on + strand at 418.436 kb on + strand at 418.437 kb on - strand at 418.437 kb on - strand at 418.469 kb on + strand at 418.469 kb on + strand at 418.469 kb on + strand at 418.469 kb on + strand at 418.469 kb on + strand at 418.469 kb on + strand at 418.469 kb on + strand at 418.469 kb on + strand at 418.469 kb on + strand at 418.470 kb on - strand at 418.470 kb on - strand at 418.470 kb on - strand at 418.610 kb on + strand at 418.611 kb on - strand at 418.652 kb on + strand, within PS417_01895 at 418.652 kb on + strand, within PS417_01895 at 418.652 kb on + strand, within PS417_01895 at 418.754 kb on + strand, within PS417_01895 at 418.985 kb on + strand, within PS417_01895 at 418.985 kb on + strand, within PS417_01895 at 418.985 kb on + strand, within PS417_01895 at 418.985 kb on + strand, within PS417_01895 at 418.985 kb on + strand, within PS417_01895 at 418.986 kb on - strand, within PS417_01895 at 418.986 kb on - strand, within PS417_01895 at 418.986 kb on - strand, within PS417_01895 at 418.986 kb on - strand, within PS417_01895 at 419.162 kb on - strand, within PS417_01895 at 419.327 kb on + strand, within PS417_01895 at 419.328 kb on - strand, within PS417_01895 at 419.328 kb on - strand, within PS417_01895 at 419.328 kb on - strand, within PS417_01895 at 419.328 kb on - strand, within PS417_01895 at 419.356 kb on + strand at 419.356 kb on + strand, within PS417_01895
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 416,869 + PS417_01885 0.13 -0.1 416,870 - PS417_01885 0.13 -0.3 416,870 - PS417_01885 0.13 +0.6 417,132 + PS417_01885 0.40 +0.8 417,621 + PS417_01885 0.89 +0.5 417,622 - PS417_01885 0.89 -0.7 417,806 + -0.2 417,807 - -0.5 417,824 + -0.0 417,825 - -1.4 417,868 + PS417_01890 0.10 +0.1 417,868 + PS417_01890 0.10 -0.7 417,868 + PS417_01890 0.10 -0.0 417,899 + PS417_01890 0.15 -0.2 417,900 - PS417_01890 0.15 +0.9 417,914 - PS417_01890 0.18 -0.8 418,152 - PS417_01890 0.56 +0.2 418,193 + PS417_01890 0.62 -0.3 418,194 - PS417_01890 0.63 -0.6 418,194 - PS417_01890 0.63 -0.0 418,277 - PS417_01890 0.76 -0.4 418,304 + PS417_01890 0.80 +0.3 418,304 + PS417_01890 0.80 +0.7 418,307 + PS417_01890 0.81 +0.5 418,436 + -0.3 418,436 + -0.3 418,437 - +0.1 418,437 - +0.2 418,469 + -0.7 418,469 + +1.1 418,469 + +0.1 418,469 + +0.7 418,469 + +1.9 418,469 + +1.3 418,469 + +0.5 418,469 + -0.1 418,469 + +1.6 418,470 - +0.0 418,470 - +0.3 418,470 - +0.1 418,610 + -0.0 418,611 - -0.1 418,652 + PS417_01895 0.12 -0.1 418,652 + PS417_01895 0.12 +0.1 418,652 + PS417_01895 0.12 +0.2 418,754 + PS417_01895 0.18 +0.7 418,985 + PS417_01895 0.32 +0.2 418,985 + PS417_01895 0.32 -0.1 418,985 + PS417_01895 0.32 -0.2 418,985 + PS417_01895 0.32 +0.1 418,985 + PS417_01895 0.32 +0.0 418,986 - PS417_01895 0.32 +0.2 418,986 - PS417_01895 0.32 +0.2 418,986 - PS417_01895 0.32 -0.8 418,986 - PS417_01895 0.32 +0.5 419,162 - PS417_01895 0.42 +0.7 419,327 + PS417_01895 0.52 +0.0 419,328 - PS417_01895 0.52 +0.3 419,328 - PS417_01895 0.52 -0.5 419,328 - PS417_01895 0.52 -0.6 419,328 - PS417_01895 0.52 +0.3 419,356 + -0.7 419,356 + PS417_01895 0.54 +0.2
Or see this region's nucleotide sequence