Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12780 and PS417_12785 are separated by 184 nucleotides PS417_12785 and PS417_12790 are separated by 120 nucleotides PS417_12790 and PS417_12795 are separated by 358 nucleotides
PS417_12780: PS417_12780 - (Fe-S)-binding protein, at 2,791,935 to 2,793,344
_12780
PS417_12785: PS417_12785 - hypothetical protein, at 2,793,529 to 2,793,768
_12785
PS417_12790: PS417_12790 - methyltransferase, at 2,793,889 to 2,794,380
_12790
PS417_12795: PS417_12795 - alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase, at 2,794,739 to 2,796,721
_12795
Position (kb)
2793
2794 Strain fitness (log2 ratio)
-1
0
1 at 2792.650 kb on - strand, within PS417_12780 at 2792.650 kb on - strand, within PS417_12780 at 2792.719 kb on + strand, within PS417_12780 at 2792.719 kb on + strand, within PS417_12780 at 2792.720 kb on - strand, within PS417_12780 at 2792.720 kb on - strand, within PS417_12780 at 2793.231 kb on - strand at 2793.258 kb on - strand at 2793.382 kb on + strand at 2793.383 kb on - strand at 2793.383 kb on - strand at 2793.624 kb on - strand, within PS417_12785 at 2793.624 kb on - strand, within PS417_12785 at 2793.624 kb on - strand at 2793.624 kb on - strand, within PS417_12785 at 2793.681 kb on + strand, within PS417_12785 at 2793.737 kb on + strand, within PS417_12785 at 2793.737 kb on + strand, within PS417_12785 at 2793.737 kb on + strand, within PS417_12785 at 2793.738 kb on - strand, within PS417_12785 at 2793.738 kb on - strand, within PS417_12785 at 2793.738 kb on - strand, within PS417_12785 at 2794.314 kb on + strand, within PS417_12790 at 2794.652 kb on - strand at 2794.654 kb on + strand at 2794.722 kb on + strand at 2794.752 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 2,792,650 - PS417_12780 0.51 -0.4 2,792,650 - PS417_12780 0.51 -1.0 2,792,719 + PS417_12780 0.56 -0.4 2,792,719 + PS417_12780 0.56 +0.8 2,792,720 - PS417_12780 0.56 +0.1 2,792,720 - PS417_12780 0.56 -0.3 2,793,231 - +0.1 2,793,258 - +0.0 2,793,382 + +0.5 2,793,383 - -0.1 2,793,383 - -0.5 2,793,624 - PS417_12785 0.40 -0.4 2,793,624 - PS417_12785 0.40 -0.7 2,793,624 - -1.5 2,793,624 - PS417_12785 0.40 -0.0 2,793,681 + PS417_12785 0.63 -0.1 2,793,737 + PS417_12785 0.87 -0.3 2,793,737 + PS417_12785 0.87 -0.3 2,793,737 + PS417_12785 0.87 -0.3 2,793,738 - PS417_12785 0.87 +0.4 2,793,738 - PS417_12785 0.87 +0.2 2,793,738 - PS417_12785 0.87 +0.3 2,794,314 + PS417_12790 0.86 +0.5 2,794,652 - -0.8 2,794,654 + -0.3 2,794,722 + -0.4 2,794,752 + +0.2
Or see this region's nucleotide sequence