Strain Fitness in Pseudomonas simiae WCS417 around PS417_10205

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
2,183,312	+		PS417_10200	0.83	-1.0
2,183,313	-		PS417_10200	0.83	-0.0
2,183,514	+		PS417_10200	0.89	+0.1
2,183,534	+		PS417_10200	0.90	+0.1
2,183,535	-		PS417_10200	0.90	+0.4
2,183,535	-				+1.7
2,183,648	+				+0.4
2,183,688	+				+0.6
2,183,854	+				-0.1
2,183,905	+				-0.3
2,183,906	-				-0.1
2,183,909	+				+0.3
2,183,910	-				-0.2
2,183,980	-				-0.3
2,183,986	+				-0.4
2,184,034	+				-0.4
2,184,045	-				+0.3
2,184,052	-				+0.1
2,184,088	+				-0.1
2,184,100	-				+0.4
2,184,100	-				-0.0
2,184,277	-				+0.3
2,184,286	+				-0.4
2,184,311	-				+1.7
2,184,398	-		PS417_10205	0.14	-0.2
2,184,421	-		PS417_10205	0.17	-0.5
2,184,526	+		PS417_10205	0.31	-0.2
2,184,528	+		PS417_10205	0.32	+0.3
2,184,529	-		PS417_10205	0.32	-1.2
2,184,529	-		PS417_10205	0.32	+0.0
2,184,534	+		PS417_10205	0.33	-0.2
2,184,639	+		PS417_10205	0.47	-1.0
2,184,735	+		PS417_10205	0.60	+0.2
2,184,961	-				-0.1
2,184,975	+				+0.6
2,184,976	-				-0.1
2,185,117	+				-0.4
2,185,118	-				+0.5
2,185,140	+				+0.1
2,185,140	+				-0.3
2,185,341	+		PS417_10210	0.42	+0.8
2,185,413	-		PS417_10210	0.56	-0.0
2,185,501	+		PS417_10210	0.73	-0.1
2,185,502	-		PS417_10210	0.73	+0.2
2,185,502	-		PS417_10210	0.73	+0.2
2,185,575	+		PS417_10210	0.87	-0.5
2,185,576	-		PS417_10210	0.87	+0.1
2,185,596	+				-0.5
2,185,596	+				+0.4
2,185,596	+				+0.3
2,185,614	+				+0.1
2,185,615	-				-0.0
2,185,615	-				+0.2
2,185,615	-				-0.2
2,185,615	-				-0.1
2,185,615	-				-0.4
2,185,649	-				-0.1
2,185,649	-				+0.1
2,185,792	-		PS417_10215	0.52	-0.1
2,185,873	+				+0.2
2,185,926	+				+0.8
2,185,927	-				-0.2