Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04925 and PS417_04930 overlap by 4 nucleotides PS417_04930 and PS417_04935 are separated by 17 nucleotides PS417_04935 and PS417_04940 are separated by 10 nucleotides
PS417_04925: PS417_04925 - MFS transporter, at 1,096,804 to 1,098,069
_04925
PS417_04930: PS417_04930 - hypothetical protein, at 1,098,066 to 1,098,704
_04930
PS417_04935: PS417_04935 - hypothetical protein, at 1,098,722 to 1,099,846
_04935
PS417_04940: PS417_04940 - outer membrane usher protein, at 1,099,857 to 1,102,298
_04940
Position (kb)
1098
1099
1100 Strain fitness (log2 ratio)
-1
0
1 at 1097.752 kb on + strand, within PS417_04925 at 1097.753 kb on - strand, within PS417_04925 at 1097.753 kb on - strand, within PS417_04925 at 1098.041 kb on - strand at 1098.067 kb on + strand at 1098.073 kb on + strand at 1098.073 kb on + strand at 1098.074 kb on - strand at 1098.074 kb on - strand at 1098.074 kb on - strand at 1098.310 kb on + strand, within PS417_04930 at 1098.409 kb on + strand, within PS417_04930 at 1098.409 kb on + strand, within PS417_04930 at 1098.410 kb on - strand at 1098.427 kb on + strand, within PS417_04930 at 1098.679 kb on - strand at 1098.730 kb on - strand at 1098.821 kb on + strand at 1099.017 kb on + strand, within PS417_04935 at 1099.017 kb on + strand, within PS417_04935 at 1099.018 kb on - strand, within PS417_04935 at 1099.020 kb on + strand, within PS417_04935 at 1100.231 kb on - strand, within PS417_04940 at 1100.389 kb on - strand, within PS417_04940 at 1100.416 kb on + strand, within PS417_04940 at 1100.447 kb on - strand, within PS417_04940 at 1100.662 kb on + strand, within PS417_04940
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 1,097,752 + PS417_04925 0.75 -0.3 1,097,753 - PS417_04925 0.75 -0.0 1,097,753 - PS417_04925 0.75 -0.0 1,098,041 - -0.2 1,098,067 + -1.1 1,098,073 + -0.1 1,098,073 + +0.5 1,098,074 - -0.2 1,098,074 - -0.0 1,098,074 - -0.1 1,098,310 + PS417_04930 0.38 -0.6 1,098,409 + PS417_04930 0.54 -0.3 1,098,409 + PS417_04930 0.54 +0.0 1,098,410 - +0.1 1,098,427 + PS417_04930 0.56 -0.3 1,098,679 - -0.1 1,098,730 - -0.0 1,098,821 + +0.4 1,099,017 + PS417_04935 0.26 +0.0 1,099,017 + PS417_04935 0.26 -0.1 1,099,018 - PS417_04935 0.26 +1.0 1,099,020 + PS417_04935 0.26 +0.7 1,100,231 - PS417_04940 0.15 +0.4 1,100,389 - PS417_04940 0.22 +0.4 1,100,416 + PS417_04940 0.23 +0.0 1,100,447 - PS417_04940 0.24 +0.5 1,100,662 + PS417_04940 0.33 -0.3
Or see this region's nucleotide sequence