Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04590 and PS417_04595 are separated by 804 nucleotides PS417_04595 and PS417_04600 overlap by 4 nucleotides PS417_04600 and PS417_04605 overlap by 4 nucleotides
PS417_04590: PS417_04590 - methyltransferase, at 1,016,127 to 1,016,999
_04590
PS417_04595: PS417_04595 - cell division protein MraZ, at 1,017,804 to 1,018,259
_04595
PS417_04600: PS417_04600 - 16S rRNA methyltransferase, at 1,018,256 to 1,019,203
_04600
PS417_04605: PS417_04605 - cell division protein FtsL, at 1,019,200 to 1,019,493
_04605
Position (kb)
1017
1018
1019 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1016.876 kb on + strand, within PS417_04590 at 1016.877 kb on - strand, within PS417_04590 at 1016.887 kb on + strand, within PS417_04590 at 1016.887 kb on + strand, within PS417_04590 at 1016.888 kb on - strand, within PS417_04590 at 1016.964 kb on + strand at 1016.964 kb on + strand at 1016.997 kb on + strand at 1016.998 kb on - strand at 1017.018 kb on - strand at 1017.053 kb on - strand at 1017.055 kb on + strand at 1017.055 kb on + strand at 1017.056 kb on - strand at 1017.056 kb on - strand at 1017.487 kb on + strand at 1017.490 kb on + strand at 1017.491 kb on - strand at 1017.599 kb on + strand at 1017.724 kb on + strand at 1017.724 kb on + strand at 1017.937 kb on + strand, within PS417_04595
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 1,016,876 + PS417_04590 0.86 +0.1 1,016,877 - PS417_04590 0.86 +0.1 1,016,887 + PS417_04590 0.87 -0.7 1,016,887 + PS417_04590 0.87 +0.8 1,016,888 - PS417_04590 0.87 +0.2 1,016,964 + +0.4 1,016,964 + -0.1 1,016,997 + +0.2 1,016,998 - -0.1 1,017,018 - -0.3 1,017,053 - -1.0 1,017,055 + +3.9 1,017,055 + +0.1 1,017,056 - -1.2 1,017,056 - -0.9 1,017,487 + +0.3 1,017,490 + -0.3 1,017,491 - +0.9 1,017,599 + +0.9 1,017,724 + +0.2 1,017,724 + +0.6 1,017,937 + PS417_04595 0.29 +0.9
Or see this region's nucleotide sequence