Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04370 and PS417_04375 are separated by 298 nucleotides PS417_04375 and PS417_04380 overlap by 1 nucleotides PS417_04380 and PS417_04385 overlap by 1 nucleotides
PS417_04370: PS417_04370 - D-arabinose 5-phosphate isomerase, at 975,087 to 976,061
_04370
PS417_04375: PS417_04375 - ABC transporter ATP-binding protein, at 976,360 to 977,169
_04375
PS417_04380: PS417_04380 - ABC transporter permease, at 977,169 to 977,966
_04380
PS417_04385: PS417_04385 - organic solvent ABC transporter substrate-binding protein, at 977,966 to 978,433
_04385
Position (kb) 976
977
978 Strain fitness (log2 ratio) -1
0
1
2 at 976.074 kb on - strand at 976.076 kb on - strand at 976.076 kb on - strand at 976.244 kb on - strand at 976.245 kb on + strand at 976.245 kb on + strand at 976.246 kb on - strand at 976.246 kb on - strand at 976.287 kb on + strand at 976.287 kb on + strand at 976.287 kb on + strand at 976.288 kb on - strand at 976.357 kb on + strand at 976.434 kb on + strand at 976.617 kb on + strand, within PS417_04375 at 976.618 kb on - strand, within PS417_04375 at 976.755 kb on - strand, within PS417_04375 at 976.862 kb on - strand, within PS417_04375 at 977.000 kb on - strand, within PS417_04375 at 977.006 kb on - strand, within PS417_04375 at 977.422 kb on - strand, within PS417_04380 at 977.422 kb on - strand, within PS417_04380 at 977.605 kb on - strand, within PS417_04380 at 977.902 kb on - strand at 978.079 kb on + strand, within PS417_04385 at 978.085 kb on + strand, within PS417_04385 at 978.098 kb on + strand, within PS417_04385 at 978.134 kb on - strand, within PS417_04385
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 976,074 - -0.0 976,076 - +0.1 976,076 - +0.2 976,244 - -1.2 976,245 + -0.3 976,245 + -0.4 976,246 - +1.3 976,246 - -0.4 976,287 + +0.2 976,287 + -0.1 976,287 + -0.3 976,288 - +0.7 976,357 + +0.2 976,434 + +1.9 976,617 + PS417_04375 0.32 +0.1 976,618 - PS417_04375 0.32 -0.7 976,755 - PS417_04375 0.49 +0.3 976,862 - PS417_04375 0.62 +1.9 977,000 - PS417_04375 0.79 +0.3 977,006 - PS417_04375 0.80 +0.9 977,422 - PS417_04380 0.32 +1.9 977,422 - PS417_04380 0.32 +0.6 977,605 - PS417_04380 0.55 +1.9 977,902 - +1.6 978,079 + PS417_04385 0.24 -0.1 978,085 + PS417_04385 0.25 +1.7 978,098 + PS417_04385 0.28 +0.3 978,134 - PS417_04385 0.36 +0.9
Or see this region's nucleotide sequence