Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00410 and PS417_00415 are separated by 192 nucleotides PS417_00415 and PS417_00420 are separated by 34 nucleotides PS417_00420 and PS417_00425 are separated by 168 nucleotides PS417_00425 and PS417_00430 are separated by 10 nucleotides
PS417_00410: PS417_00410 - GTP-binding protein, at 81,025 to 81,666
_00410
PS417_00415: PS417_00415 - cytochrome, at 81,859 to 82,161
_00415
PS417_00420: PS417_00420 - cytochrome C, at 82,196 to 82,822
_00420
PS417_00425: PS417_00425 - thiol:disulfide interchange protein, at 82,991 to 83,626
_00425
PS417_00430: PS417_00430 - endonuclease, at 83,637 to 84,515
_00430
Position (kb) 82
83 Strain fitness (log2 ratio) -1
0
1
2 at 81.740 kb on + strand at 81.740 kb on + strand at 81.740 kb on + strand at 81.740 kb on + strand at 81.742 kb on + strand at 81.809 kb on + strand at 81.809 kb on + strand at 81.937 kb on + strand, within PS417_00415 at 82.159 kb on + strand at 82.159 kb on + strand at 82.159 kb on + strand at 82.166 kb on + strand at 82.821 kb on - strand at 82.832 kb on - strand at 82.849 kb on - strand at 82.868 kb on - strand at 82.971 kb on + strand at 82.985 kb on + strand at 83.624 kb on + strand at 83.790 kb on + strand, within PS417_00430 at 83.791 kb on - strand, within PS417_00430 at 83.791 kb on - strand, within PS417_00430 at 83.791 kb on - strand, within PS417_00430
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 81,740 + +0.1 81,740 + -0.2 81,740 + +0.4 81,740 + +0.5 81,742 + -0.4 81,809 + +0.4 81,809 + -0.4 81,937 + PS417_00415 0.26 +2.0 82,159 + +1.0 82,159 + +0.1 82,159 + +1.2 82,166 + +0.2 82,821 - +0.4 82,832 - -0.1 82,849 - -0.3 82,868 - +0.7 82,971 + -0.3 82,985 + +0.4 83,624 + +0.3 83,790 + PS417_00430 0.17 +0.3 83,791 - PS417_00430 0.18 -0.1 83,791 - PS417_00430 0.18 -0.4 83,791 - PS417_00430 0.18 +0.1
Or see this region's nucleotide sequence