Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00405 and PS417_00410 are separated by 269 nucleotides PS417_00410 and PS417_00415 are separated by 192 nucleotides PS417_00415 and PS417_00420 are separated by 34 nucleotides
PS417_00405: PS417_00405 - DNA polymerase I, at 77,978 to 80,755
_00405
PS417_00410: PS417_00410 - GTP-binding protein, at 81,025 to 81,666
_00410
PS417_00415: PS417_00415 - cytochrome, at 81,859 to 82,161
_00415
PS417_00420: PS417_00420 - cytochrome C, at 82,196 to 82,822
_00420
Position (kb) 81
82 Strain fitness (log2 ratio) -1
0
1
2 at 80.070 kb on + strand, within PS417_00405 at 80.330 kb on - strand, within PS417_00405 at 80.339 kb on + strand, within PS417_00405 at 80.339 kb on + strand, within PS417_00405 at 80.369 kb on + strand, within PS417_00405 at 80.370 kb on - strand, within PS417_00405 at 80.370 kb on - strand, within PS417_00405 at 80.408 kb on + strand, within PS417_00405 at 80.799 kb on - strand at 80.829 kb on + strand at 80.853 kb on + strand at 80.854 kb on - strand at 80.911 kb on + strand at 80.911 kb on + strand at 80.911 kb on + strand at 80.911 kb on + strand at 80.912 kb on - strand at 80.912 kb on - strand at 80.912 kb on - strand at 80.921 kb on + strand at 80.921 kb on + strand at 80.921 kb on + strand at 80.936 kb on - strand at 81.740 kb on + strand at 81.740 kb on + strand at 81.740 kb on + strand at 81.740 kb on + strand at 81.742 kb on + strand at 81.809 kb on + strand at 81.809 kb on + strand at 81.937 kb on + strand, within PS417_00415 at 82.159 kb on + strand at 82.159 kb on + strand at 82.159 kb on + strand at 82.166 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 80,070 + PS417_00405 0.75 +1.4 80,330 - PS417_00405 0.85 +1.1 80,339 + PS417_00405 0.85 +0.8 80,339 + PS417_00405 0.85 +0.9 80,369 + PS417_00405 0.86 +1.3 80,370 - PS417_00405 0.86 +1.0 80,370 - PS417_00405 0.86 +1.2 80,408 + PS417_00405 0.87 +2.2 80,799 - -0.2 80,829 + -0.2 80,853 + -0.4 80,854 - -0.1 80,911 + -0.2 80,911 + -0.2 80,911 + +0.7 80,911 + +0.7 80,912 - -0.3 80,912 - +0.5 80,912 - -0.6 80,921 + -0.4 80,921 + +0.1 80,921 + +0.0 80,936 - -0.1 81,740 + +0.1 81,740 + -0.2 81,740 + +0.4 81,740 + +0.5 81,742 + -0.4 81,809 + +0.4 81,809 + -0.4 81,937 + PS417_00415 0.26 +2.0 82,159 + +1.0 82,159 + +0.1 82,159 + +1.2 82,166 + +0.2
Or see this region's nucleotide sequence