Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03730 and PS417_03735 are separated by 85 nucleotides PS417_03735 and PS417_03740 are separated by 91 nucleotides PS417_03740 and PS417_03745 are separated by 255 nucleotides PS417_03745 and PS417_03750 are separated by 253 nucleotides
PS417_03730: PS417_03730 - phosphate acetyltransferase, at 844,773 to 846,872
_03730
PS417_03735: PS417_03735 - hypothetical protein, at 846,958 to 847,149
_03735
PS417_03740: PS417_03740 - peptidylprolyl isomerase, at 847,241 to 847,726
_03740
PS417_03745: PS417_03745 - 30S ribosomal protein S20, at 847,982 to 848,260
_03745
PS417_03750: PS417_03750 - membrane protein, at 848,514 to 850,052
_03750
Position (kb)
847
848 Strain fitness (log2 ratio)
-1
0
1 at 846.910 kb on + strand at 846.910 kb on + strand at 846.910 kb on + strand at 846.910 kb on + strand at 846.910 kb on + strand at 846.911 kb on - strand at 847.277 kb on + strand at 847.278 kb on - strand at 847.280 kb on - strand at 847.280 kb on - strand at 847.340 kb on + strand, within PS417_03740 at 847.357 kb on + strand, within PS417_03740 at 847.358 kb on - strand, within PS417_03740 at 847.358 kb on - strand, within PS417_03740 at 847.425 kb on + strand, within PS417_03740 at 847.452 kb on - strand, within PS417_03740 at 847.742 kb on + strand at 847.742 kb on + strand at 847.746 kb on + strand at 847.746 kb on + strand at 847.851 kb on + strand at 847.852 kb on - strand at 847.852 kb on - strand at 847.852 kb on - strand at 847.852 kb on - strand at 847.852 kb on - strand at 848.394 kb on - strand at 848.463 kb on - strand at 848.495 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 846,910 + -0.5 846,910 + +0.0 846,910 + -0.8 846,910 + +0.7 846,910 + -0.4 846,911 - +0.1 847,277 + -0.4 847,278 - +0.4 847,280 - -0.1 847,280 - -0.5 847,340 + PS417_03740 0.20 -0.6 847,357 + PS417_03740 0.24 -0.2 847,358 - PS417_03740 0.24 +1.0 847,358 - PS417_03740 0.24 -0.0 847,425 + PS417_03740 0.38 +1.3 847,452 - PS417_03740 0.43 +0.3 847,742 + -0.0 847,742 + -0.2 847,746 + +0.5 847,746 + +1.0 847,851 + -0.1 847,852 - -0.0 847,852 - -0.1 847,852 - +0.4 847,852 - +0.1 847,852 - -0.2 848,394 - -0.4 848,463 - +0.1 848,495 - +0.2
Or see this region's nucleotide sequence