Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03305 and PS417_03310 are separated by 139 nucleotides PS417_03310 and PS417_03315 overlap by 4 nucleotides PS417_03315 and PS417_03320 are separated by 143 nucleotides
PS417_03305: PS417_03305 - HPr, at 757,694 to 758,098
_03305
PS417_03310: PS417_03310 - carbonate dehydratase, at 758,238 to 758,882
_03310
PS417_03315: PS417_03315 - hypothetical protein, at 758,879 to 759,406
_03315
PS417_03320: PS417_03320 - hypothetical protein, at 759,550 to 760,719
_03320
Position (kb) 758
759
760 Strain fitness (log2 ratio) -1
0
1 at 758.123 kb on + strand at 758.125 kb on + strand at 758.125 kb on + strand at 758.125 kb on + strand at 758.126 kb on - strand at 758.126 kb on - strand at 758.126 kb on - strand at 758.248 kb on + strand at 758.248 kb on + strand at 758.347 kb on - strand, within PS417_03310 at 758.347 kb on - strand, within PS417_03310 at 758.532 kb on + strand, within PS417_03310 at 758.617 kb on - strand, within PS417_03310 at 758.619 kb on + strand, within PS417_03310 at 758.781 kb on + strand, within PS417_03310 at 758.782 kb on - strand, within PS417_03310 at 758.782 kb on - strand, within PS417_03310 at 758.880 kb on + strand at 759.150 kb on + strand, within PS417_03315 at 759.208 kb on - strand, within PS417_03315 at 759.230 kb on + strand, within PS417_03315 at 759.230 kb on + strand, within PS417_03315 at 759.231 kb on - strand, within PS417_03315 at 759.231 kb on - strand, within PS417_03315 at 759.291 kb on - strand, within PS417_03315 at 759.291 kb on - strand, within PS417_03315 at 759.291 kb on - strand, within PS417_03315 at 759.291 kb on - strand, within PS417_03315 at 759.379 kb on + strand at 759.380 kb on - strand at 759.406 kb on + strand at 759.406 kb on + strand at 759.407 kb on - strand at 759.420 kb on + strand at 759.421 kb on - strand at 759.452 kb on - strand at 759.509 kb on + strand at 759.678 kb on - strand, within PS417_03320 at 759.858 kb on - strand, within PS417_03320 at 759.858 kb on - strand, within PS417_03320 at 759.879 kb on - strand, within PS417_03320 at 760.076 kb on + strand, within PS417_03320 at 760.088 kb on + strand, within PS417_03320 at 760.266 kb on - strand, within PS417_03320 at 760.344 kb on + strand, within PS417_03320 at 760.344 kb on + strand, within PS417_03320 at 760.345 kb on - strand, within PS417_03320 at 760.346 kb on + strand, within PS417_03320
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 758,123 + +1.1 758,125 + -0.2 758,125 + +0.4 758,125 + -0.1 758,126 - -0.5 758,126 - -0.9 758,126 - +0.0 758,248 + +0.5 758,248 + -0.4 758,347 - PS417_03310 0.17 +0.3 758,347 - PS417_03310 0.17 -0.1 758,532 + PS417_03310 0.46 -0.1 758,617 - PS417_03310 0.59 -0.3 758,619 + PS417_03310 0.59 -0.1 758,781 + PS417_03310 0.84 +0.1 758,782 - PS417_03310 0.84 -0.1 758,782 - PS417_03310 0.84 -0.5 758,880 + +0.2 759,150 + PS417_03315 0.51 +0.9 759,208 - PS417_03315 0.62 -0.4 759,230 + PS417_03315 0.66 +0.3 759,230 + PS417_03315 0.66 +0.5 759,231 - PS417_03315 0.67 +1.0 759,231 - PS417_03315 0.67 -0.1 759,291 - PS417_03315 0.78 +0.0 759,291 - PS417_03315 0.78 +0.0 759,291 - PS417_03315 0.78 +0.0 759,291 - PS417_03315 0.78 +0.3 759,379 + +0.5 759,380 - -0.3 759,406 + -0.1 759,406 + +0.1 759,407 - +0.1 759,420 + -0.0 759,421 - -0.9 759,452 - -0.2 759,509 + -0.1 759,678 - PS417_03320 0.11 +0.1 759,858 - PS417_03320 0.26 -0.2 759,858 - PS417_03320 0.26 +0.2 759,879 - PS417_03320 0.28 -0.1 760,076 + PS417_03320 0.45 +0.0 760,088 + PS417_03320 0.46 -0.2 760,266 - PS417_03320 0.61 +0.0 760,344 + PS417_03320 0.68 -0.2 760,344 + PS417_03320 0.68 -0.3 760,345 - PS417_03320 0.68 +0.2 760,346 + PS417_03320 0.68 +0.1
Or see this region's nucleotide sequence