Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02885 and PS417_02890 are separated by 262 nucleotides PS417_02890 and PS417_02895 overlap by 4 nucleotides PS417_02895 and PS417_02900 are separated by 38 nucleotides
PS417_02885: PS417_02885 - hypothetical protein, at 659,193 to 659,588
_02885
PS417_02890: PS417_02890 - cobalt-precorrin-6X reductase, at 659,851 to 660,570
_02890
PS417_02895: PS417_02895 - precorrin-6Y C5,15-methyltransferase, at 660,567 to 661,772
_02895
PS417_02900: PS417_02900 - oxidoreductase, at 661,811 to 663,157
_02900
Position (kb)
660
661
662 Strain fitness (log2 ratio)
-1
0
1 at 659.629 kb on - strand at 659.651 kb on - strand at 659.651 kb on - strand at 659.651 kb on - strand at 659.743 kb on - strand at 659.743 kb on - strand at 659.743 kb on - strand at 660.494 kb on + strand, within PS417_02890 at 660.494 kb on + strand, within PS417_02890 at 660.494 kb on + strand, within PS417_02890 at 660.498 kb on + strand, within PS417_02890 at 660.498 kb on + strand, within PS417_02890 at 660.498 kb on + strand, within PS417_02890 at 660.499 kb on - strand at 660.499 kb on - strand at 660.517 kb on - strand at 661.334 kb on + strand, within PS417_02895 at 661.334 kb on + strand, within PS417_02895 at 661.532 kb on - strand, within PS417_02895 at 661.676 kb on + strand at 661.750 kb on - strand at 661.750 kb on - strand at 661.772 kb on + strand at 661.772 kb on + strand at 661.773 kb on - strand at 661.777 kb on - strand at 661.777 kb on - strand at 661.777 kb on - strand at 661.777 kb on - strand at 661.777 kb on - strand at 661.834 kb on - strand at 661.834 kb on - strand at 661.892 kb on - strand at 662.027 kb on + strand, within PS417_02900 at 662.651 kb on + strand, within PS417_02900
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 659,629 - -0.1 659,651 - -0.1 659,651 - +0.2 659,651 - +0.0 659,743 - +0.5 659,743 - +0.6 659,743 - -0.1 660,494 + PS417_02890 0.89 -1.1 660,494 + PS417_02890 0.89 -0.0 660,494 + PS417_02890 0.89 +1.2 660,498 + PS417_02890 0.90 +0.3 660,498 + PS417_02890 0.90 +1.1 660,498 + PS417_02890 0.90 -0.5 660,499 - -0.4 660,499 - -0.3 660,517 - +0.1 661,334 + PS417_02895 0.64 +0.8 661,334 + PS417_02895 0.64 +0.2 661,532 - PS417_02895 0.80 +0.3 661,676 + -0.0 661,750 - -0.9 661,750 - +0.2 661,772 + -0.1 661,772 + +0.4 661,773 - +0.1 661,777 - -0.3 661,777 - -0.5 661,777 - +0.0 661,777 - -0.4 661,777 - -0.1 661,834 - -1.1 661,834 - -0.1 661,892 - -0.2 662,027 + PS417_02900 0.16 +0.9 662,651 + PS417_02900 0.62 +1.5
Or see this region's nucleotide sequence