Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_28185 and PS417_28190 are separated by 112 nucleotides PS417_28190 and PS417_28195 overlap by 4 nucleotides PS417_28195 and PS417_28200 are separated by 3 nucleotides
PS417_28185: PS417_28185 - homocysteine methyltransferase, at 6,085,661 to 6,086,557
_28185
PS417_28190: PS417_28190 - amino acid ABC transporter substrate-binding protein, at 6,086,670 to 6,087,491
_28190
PS417_28195: PS417_28195 - amino acid ABC transporter permease, at 6,087,488 to 6,088,318
_28195
PS417_28200: PS417_28200 - hypothetical protein, at 6,088,322 to 6,088,567
_28200
Position (kb)
6086
6087
6088 Strain fitness (log2 ratio)
-1
0
1 at 6085.768 kb on - strand, within PS417_28185 at 6086.227 kb on - strand, within PS417_28185 at 6086.379 kb on + strand, within PS417_28185 at 6086.418 kb on + strand, within PS417_28185 at 6086.418 kb on + strand, within PS417_28185 at 6086.418 kb on + strand, within PS417_28185 at 6086.419 kb on - strand, within PS417_28185 at 6086.540 kb on + strand at 6086.540 kb on + strand at 6086.540 kb on + strand at 6086.567 kb on - strand at 6086.615 kb on + strand at 6087.016 kb on - strand, within PS417_28190 at 6087.130 kb on - strand, within PS417_28190 at 6087.137 kb on + strand, within PS417_28190 at 6087.181 kb on - strand, within PS417_28190 at 6087.211 kb on - strand, within PS417_28190 at 6087.348 kb on + strand, within PS417_28190 at 6087.872 kb on + strand, within PS417_28195 at 6087.872 kb on + strand, within PS417_28195 at 6087.873 kb on - strand, within PS417_28195 at 6087.873 kb on - strand, within PS417_28195 at 6087.944 kb on + strand, within PS417_28195 at 6087.945 kb on - strand, within PS417_28195 at 6088.182 kb on - strand, within PS417_28195 at 6088.182 kb on - strand, within PS417_28195 at 6088.202 kb on + strand, within PS417_28195 at 6088.202 kb on + strand, within PS417_28195 at 6088.203 kb on - strand, within PS417_28195 at 6088.235 kb on + strand, within PS417_28195 at 6088.236 kb on - strand at 6088.236 kb on - strand at 6088.301 kb on + strand at 6088.301 kb on + strand at 6088.469 kb on - strand, within PS417_28200 at 6088.469 kb on - strand, within PS417_28200
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 6,085,768 - PS417_28185 0.12 +0.6 6,086,227 - PS417_28185 0.63 +0.3 6,086,379 + PS417_28185 0.80 -0.1 6,086,418 + PS417_28185 0.84 +0.5 6,086,418 + PS417_28185 0.84 -0.5 6,086,418 + PS417_28185 0.84 +0.4 6,086,419 - PS417_28185 0.85 -0.5 6,086,540 + -0.1 6,086,540 + -0.1 6,086,540 + -0.8 6,086,567 - +0.0 6,086,615 + +0.0 6,087,016 - PS417_28190 0.42 +0.6 6,087,130 - PS417_28190 0.56 +0.1 6,087,137 + PS417_28190 0.57 +0.2 6,087,181 - PS417_28190 0.62 +1.2 6,087,211 - PS417_28190 0.66 +0.4 6,087,348 + PS417_28190 0.82 +1.7 6,087,872 + PS417_28195 0.46 -0.3 6,087,872 + PS417_28195 0.46 -0.0 6,087,873 - PS417_28195 0.46 -0.1 6,087,873 - PS417_28195 0.46 -0.0 6,087,944 + PS417_28195 0.55 -0.2 6,087,945 - PS417_28195 0.55 -0.2 6,088,182 - PS417_28195 0.84 +0.4 6,088,182 - PS417_28195 0.84 -0.0 6,088,202 + PS417_28195 0.86 +0.0 6,088,202 + PS417_28195 0.86 +0.2 6,088,203 - PS417_28195 0.86 +0.2 6,088,235 + PS417_28195 0.90 +1.0 6,088,236 - -0.1 6,088,236 - +0.2 6,088,301 + +0.3 6,088,301 + -0.1 6,088,469 - PS417_28200 0.60 -0.4 6,088,469 - PS417_28200 0.60 -0.3
Or see this region's nucleotide sequence