Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24020 and PS417_24025 are separated by 106 nucleotides PS417_24025 and PS417_24030 are separated by 9 nucleotides PS417_24030 and PS417_24035 are separated by 28 nucleotides PS417_24035 and PS417_24040 overlap by 4 nucleotides
PS417_24020: PS417_24020 - 23S rRNA methyltransferase, at 5,200,956 to 5,201,606
_24020
PS417_24025: PS417_24025 - RNA-binding protein, at 5,201,713 to 5,202,021
_24025
PS417_24030: PS417_24030 - MFS transporter, at 5,202,031 to 5,202,435
_24030
PS417_24035: PS417_24035 - transcription elongation factor GreA, at 5,202,464 to 5,202,940
_24035
PS417_24040: PS417_24040 - carbamoyl phosphate synthase large subunit, at 5,202,937 to 5,206,158
_24040
Position (kb) 5202
5203 Strain fitness (log2 ratio) -1
0
1 at 5202.006 kb on - strand at 5202.006 kb on - strand at 5202.117 kb on - strand, within PS417_24030 at 5202.125 kb on - strand, within PS417_24030 at 5202.171 kb on - strand, within PS417_24030 at 5202.186 kb on - strand, within PS417_24030 at 5202.773 kb on - strand, within PS417_24035 at 5202.959 kb on - strand at 5202.959 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 5,202,006 - -0.0 5,202,006 - +0.3 5,202,117 - PS417_24030 0.21 -1.7 5,202,125 - PS417_24030 0.23 -0.4 5,202,171 - PS417_24030 0.35 -0.0 5,202,186 - PS417_24030 0.38 -0.1 5,202,773 - PS417_24035 0.65 +0.9 5,202,959 - -1.1 5,202,959 - -0.1
Or see this region's nucleotide sequence