Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02145 and PS417_02150 are separated by 50 nucleotides PS417_02150 and PS417_02155 are separated by 243 nucleotides
PS417_02145: PS417_02145 - potassium transporter KefA, at 477,221 to 480,559
_02145
PS417_02150: PS417_02150 - potassium/proton antiporter, at 480,610 to 482,352
_02150
PS417_02155: PS417_02155 - chemotaxis protein, at 482,596 to 484,221
_02155
Position (kb) 480
481
482
483 Strain fitness (log2 ratio) -1
0
1 at 479.712 kb on + strand, within PS417_02145 at 479.713 kb on - strand, within PS417_02145 at 479.891 kb on + strand, within PS417_02145 at 479.891 kb on + strand, within PS417_02145 at 479.891 kb on + strand, within PS417_02145 at 479.892 kb on - strand, within PS417_02145 at 480.373 kb on - strand at 480.373 kb on - strand at 480.373 kb on - strand at 480.554 kb on - strand at 480.559 kb on + strand at 480.559 kb on + strand at 480.611 kb on + strand at 480.982 kb on - strand, within PS417_02150 at 481.265 kb on + strand, within PS417_02150 at 481.266 kb on - strand, within PS417_02150 at 481.350 kb on + strand, within PS417_02150 at 481.471 kb on + strand, within PS417_02150 at 481.472 kb on - strand, within PS417_02150 at 481.513 kb on - strand, within PS417_02150 at 481.513 kb on - strand, within PS417_02150 at 481.548 kb on + strand, within PS417_02150 at 481.549 kb on - strand, within PS417_02150 at 481.689 kb on - strand, within PS417_02150 at 481.689 kb on - strand, within PS417_02150 at 481.963 kb on - strand, within PS417_02150 at 482.187 kb on + strand at 482.251 kb on + strand at 482.252 kb on - strand at 482.253 kb on + strand at 482.253 kb on + strand at 482.254 kb on - strand at 482.254 kb on - strand at 482.359 kb on + strand at 482.420 kb on + strand at 482.598 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 479,712 + PS417_02145 0.75 -0.0 479,713 - PS417_02145 0.75 -0.6 479,891 + PS417_02145 0.80 -0.1 479,891 + PS417_02145 0.80 +0.0 479,891 + PS417_02145 0.80 +0.5 479,892 - PS417_02145 0.80 -0.1 480,373 - +0.5 480,373 - +0.3 480,373 - +0.2 480,554 - -0.2 480,559 + +0.0 480,559 + -0.3 480,611 + -0.3 480,982 - PS417_02150 0.21 -0.0 481,265 + PS417_02150 0.38 +0.5 481,266 - PS417_02150 0.38 -0.0 481,350 + PS417_02150 0.42 -0.1 481,471 + PS417_02150 0.49 +1.5 481,472 - PS417_02150 0.49 +0.5 481,513 - PS417_02150 0.52 +0.2 481,513 - PS417_02150 0.52 -0.3 481,548 + PS417_02150 0.54 +0.2 481,549 - PS417_02150 0.54 +0.1 481,689 - PS417_02150 0.62 +0.2 481,689 - PS417_02150 0.62 +0.3 481,963 - PS417_02150 0.78 +0.7 482,187 + +0.5 482,251 + +0.8 482,252 - -0.3 482,253 + +0.2 482,253 + +0.1 482,254 - +0.7 482,254 - +0.3 482,359 + -0.1 482,420 + -0.1 482,598 - +0.2
Or see this region's nucleotide sequence