Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_19330 and PS417_19335 are separated by 239 nucleotides PS417_19335 and PS417_19340 are separated by 142 nucleotides PS417_19340 and PS417_19345 are separated by 75 nucleotides
PS417_19330: PS417_19330 - SAM-dependent methyltransferase, at 4,192,061 to 4,192,972
_19330
PS417_19335: PS417_19335 - isochorismatase, at 4,193,212 to 4,193,802
_19335
PS417_19340: PS417_19340 - hypothetical protein, at 4,193,945 to 4,194,265
_19340
PS417_19345: PS417_19345 - DNA mismatch repair protein MutS, at 4,194,341 to 4,194,898
_19345
Position (kb) 4193
4194 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4192.717 kb on - strand, within PS417_19330 at 4193.008 kb on + strand at 4193.036 kb on - strand at 4193.036 kb on - strand at 4193.036 kb on - strand at 4193.036 kb on - strand at 4193.132 kb on + strand at 4193.132 kb on + strand at 4193.132 kb on + strand at 4193.133 kb on - strand at 4193.312 kb on - strand, within PS417_19335 at 4193.423 kb on + strand, within PS417_19335 at 4193.423 kb on + strand, within PS417_19335 at 4193.877 kb on - strand at 4193.903 kb on - strand at 4193.903 kb on - strand at 4194.045 kb on + strand, within PS417_19340 at 4194.181 kb on - strand, within PS417_19340 at 4194.312 kb on + strand at 4194.313 kb on - strand at 4194.313 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 4,192,717 - PS417_19330 0.72 +0.1 4,193,008 + +0.1 4,193,036 - -0.1 4,193,036 - -0.1 4,193,036 - +0.1 4,193,036 - -0.1 4,193,132 + -0.3 4,193,132 + -0.1 4,193,132 + -0.5 4,193,133 - -0.2 4,193,312 - PS417_19335 0.17 +0.4 4,193,423 + PS417_19335 0.36 -0.1 4,193,423 + PS417_19335 0.36 +0.7 4,193,877 - -0.2 4,193,903 - -0.4 4,193,903 - -2.8 4,194,045 + PS417_19340 0.31 +0.7 4,194,181 - PS417_19340 0.74 -1.4 4,194,312 + -0.1 4,194,313 - +1.4 4,194,313 - +0.1
Or see this region's nucleotide sequence