Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17800 and PS417_17805 overlap by 14 nucleotides PS417_17805 and PS417_17810 overlap by 4 nucleotides PS417_17810 and PS417_17815 are separated by 869 nucleotides
PS417_17800: PS417_17800 - microcin B17-processing protein McbD, at 3,886,035 to 3,887,228
_17800
PS417_17805: PS417_17805 - microcin B17 processing protein McbC, at 3,887,215 to 3,888,021
_17805
PS417_17810: PS417_17810 - microcin B17-processing protein McbB, at 3,888,018 to 3,888,875
_17810
PS417_17815: PS417_17815 - hypothetical protein, at 3,889,745 to 3,889,975
_17815
Position (kb)
3888
3889 Strain fitness (log2 ratio)
-2
-1
0
1 at 3887.068 kb on - strand, within PS417_17800 at 3887.068 kb on - strand, within PS417_17800 at 3887.068 kb on - strand, within PS417_17800 at 3887.100 kb on + strand, within PS417_17800 at 3887.185 kb on - strand at 3887.442 kb on + strand, within PS417_17805 at 3887.443 kb on - strand, within PS417_17805 at 3887.445 kb on - strand, within PS417_17805 at 3887.488 kb on - strand at 3887.713 kb on - strand at 3887.713 kb on - strand, within PS417_17805 at 3888.048 kb on - strand at 3888.132 kb on - strand, within PS417_17810 at 3888.132 kb on - strand, within PS417_17810 at 3888.353 kb on + strand, within PS417_17810 at 3888.355 kb on + strand, within PS417_17810 at 3888.483 kb on + strand, within PS417_17810 at 3888.540 kb on - strand, within PS417_17810 at 3888.617 kb on + strand, within PS417_17810 at 3888.869 kb on + strand at 3888.966 kb on + strand at 3888.967 kb on - strand at 3888.977 kb on + strand at 3889.002 kb on - strand at 3889.543 kb on + strand at 3889.690 kb on + strand at 3889.691 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 3,887,068 - PS417_17800 0.87 +0.0 3,887,068 - PS417_17800 0.87 -0.1 3,887,068 - PS417_17800 0.87 +0.2 3,887,100 + PS417_17800 0.89 +0.1 3,887,185 - +0.2 3,887,442 + PS417_17805 0.28 +0.6 3,887,443 - PS417_17805 0.28 -0.3 3,887,445 - PS417_17805 0.29 -1.9 3,887,488 - +0.0 3,887,713 - +0.0 3,887,713 - PS417_17805 0.62 -0.6 3,888,048 - +1.1 3,888,132 - PS417_17810 0.13 +0.4 3,888,132 - PS417_17810 0.13 -0.1 3,888,353 + PS417_17810 0.39 +0.3 3,888,355 + PS417_17810 0.39 +0.3 3,888,483 + PS417_17810 0.54 -0.3 3,888,540 - PS417_17810 0.61 +1.1 3,888,617 + PS417_17810 0.70 +0.5 3,888,869 + -0.2 3,888,966 + -0.2 3,888,967 - +0.2 3,888,977 + +0.6 3,889,002 - -0.4 3,889,543 + -0.6 3,889,690 + -0.1 3,889,691 - -0.9
Or see this region's nucleotide sequence