Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17745 and PS417_17750 are separated by 48 nucleotides PS417_17750 and PS417_17755 are separated by 125 nucleotides
PS417_17745: PS417_17745 - gluconolactonase, at 3,873,127 to 3,874,227
_17745
PS417_17750: PS417_17750 - hypothetical protein, at 3,874,276 to 3,875,718
_17750
PS417_17755: PS417_17755 - hypothetical protein, at 3,875,844 to 3,876,197
_17755
Position (kb)
3874
3875
3876 Strain fitness (log2 ratio)
-1
0
1
2 at 3873.550 kb on - strand, within PS417_17745 at 3873.613 kb on + strand, within PS417_17745 at 3873.619 kb on + strand, within PS417_17745 at 3873.620 kb on - strand, within PS417_17745 at 3874.117 kb on + strand, within PS417_17745 at 3874.144 kb on + strand at 3874.225 kb on + strand at 3874.260 kb on + strand at 3874.260 kb on + strand at 3874.260 kb on + strand at 3874.261 kb on - strand at 3874.261 kb on - strand at 3874.264 kb on + strand at 3874.264 kb on + strand at 3874.264 kb on + strand at 3874.265 kb on - strand at 3874.320 kb on - strand at 3874.371 kb on + strand at 3874.410 kb on - strand at 3874.633 kb on - strand, within PS417_17750 at 3874.672 kb on + strand, within PS417_17750 at 3874.923 kb on - strand, within PS417_17750 at 3875.058 kb on - strand, within PS417_17750 at 3875.129 kb on + strand, within PS417_17750 at 3875.129 kb on + strand, within PS417_17750 at 3875.130 kb on - strand, within PS417_17750 at 3875.136 kb on - strand, within PS417_17750 at 3875.139 kb on - strand, within PS417_17750 at 3875.361 kb on - strand, within PS417_17750 at 3875.697 kb on + strand at 3875.733 kb on + strand at 3875.734 kb on - strand at 3875.734 kb on - strand at 3875.782 kb on + strand at 3875.782 kb on + strand at 3875.821 kb on - strand at 3875.900 kb on - strand, within PS417_17755 at 3875.928 kb on + strand, within PS417_17755 at 3876.024 kb on - strand, within PS417_17755 at 3876.178 kb on + strand at 3876.281 kb on + strand at 3876.389 kb on + strand at 3876.390 kb on - strand at 3876.390 kb on - strand at 3876.459 kb on + strand at 3876.552 kb on + strand at 3876.552 kb on + strand at 3876.553 kb on - strand at 3876.553 kb on - strand at 3876.553 kb on - strand at 3876.636 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 3,873,550 - PS417_17745 0.38 -0.3 3,873,613 + PS417_17745 0.44 -0.1 3,873,619 + PS417_17745 0.45 +0.1 3,873,620 - PS417_17745 0.45 -0.3 3,874,117 + PS417_17745 0.90 +0.3 3,874,144 + -0.2 3,874,225 + +0.1 3,874,260 + +0.6 3,874,260 + +0.3 3,874,260 + -0.0 3,874,261 - +0.6 3,874,261 - -0.3 3,874,264 + -0.7 3,874,264 + -0.1 3,874,264 + -0.1 3,874,265 - +0.2 3,874,320 - -0.0 3,874,371 + -0.2 3,874,410 - +0.4 3,874,633 - PS417_17750 0.25 +0.1 3,874,672 + PS417_17750 0.27 +0.3 3,874,923 - PS417_17750 0.45 +0.6 3,875,058 - PS417_17750 0.54 +2.7 3,875,129 + PS417_17750 0.59 +0.7 3,875,129 + PS417_17750 0.59 +0.2 3,875,130 - PS417_17750 0.59 +0.4 3,875,136 - PS417_17750 0.60 +0.2 3,875,139 - PS417_17750 0.60 +0.8 3,875,361 - PS417_17750 0.75 -0.0 3,875,697 + +0.2 3,875,733 + +0.2 3,875,734 - +0.1 3,875,734 - +0.0 3,875,782 + +0.4 3,875,782 + +0.4 3,875,821 - -0.1 3,875,900 - PS417_17755 0.16 +0.3 3,875,928 + PS417_17755 0.24 -0.6 3,876,024 - PS417_17755 0.51 +0.2 3,876,178 + +0.6 3,876,281 + +0.1 3,876,389 + -0.5 3,876,390 - -0.0 3,876,390 - -0.1 3,876,459 + +1.1 3,876,552 + +0.2 3,876,552 + +0.0 3,876,553 - +0.3 3,876,553 - -0.2 3,876,553 - +0.0 3,876,636 + +0.9
Or see this region's nucleotide sequence