Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17115 and PS417_17120 are separated by 39 nucleotides PS417_17120 and PS417_17125 are separated by 116 nucleotides PS417_17125 and PS417_17130 are separated by 109 nucleotides
PS417_17115: PS417_17115 - ATPase, at 3,737,137 to 3,738,231
_17115
PS417_17120: PS417_17120 - protein kinase, at 3,738,271 to 3,739,563
_17120
PS417_17125: PS417_17125 - transcriptional regulator, at 3,739,680 to 3,740,387
_17125
PS417_17130: PS417_17130 - MFS transporter, at 3,740,497 to 3,741,822
_17130
Position (kb)
3738
3739
3740 Strain fitness (log2 ratio)
-1
0
1 at 3737.455 kb on - strand, within PS417_17115 at 3737.636 kb on - strand, within PS417_17115 at 3737.669 kb on - strand, within PS417_17115 at 3737.971 kb on + strand, within PS417_17115 at 3737.972 kb on - strand, within PS417_17115 at 3737.972 kb on - strand, within PS417_17115 at 3737.992 kb on + strand at 3737.992 kb on + strand, within PS417_17115 at 3738.060 kb on + strand, within PS417_17115 at 3738.083 kb on - strand, within PS417_17115 at 3738.083 kb on - strand, within PS417_17115 at 3738.276 kb on + strand at 3738.809 kb on - strand, within PS417_17120 at 3738.976 kb on + strand, within PS417_17120 at 3738.976 kb on + strand, within PS417_17120 at 3739.183 kb on + strand, within PS417_17120 at 3739.184 kb on - strand, within PS417_17120 at 3739.184 kb on - strand, within PS417_17120 at 3739.291 kb on + strand, within PS417_17120 at 3739.302 kb on + strand, within PS417_17120 at 3739.681 kb on + strand at 3739.801 kb on + strand, within PS417_17125 at 3739.801 kb on + strand, within PS417_17125 at 3739.802 kb on - strand, within PS417_17125 at 3740.075 kb on + strand, within PS417_17125 at 3740.140 kb on + strand, within PS417_17125 at 3740.360 kb on + strand at 3740.452 kb on - strand at 3740.470 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 3,737,455 - PS417_17115 0.29 +0.2 3,737,636 - PS417_17115 0.46 -0.1 3,737,669 - PS417_17115 0.49 -0.1 3,737,971 + PS417_17115 0.76 -0.6 3,737,972 - PS417_17115 0.76 -0.0 3,737,972 - PS417_17115 0.76 -0.2 3,737,992 + +1.7 3,737,992 + PS417_17115 0.78 +0.8 3,738,060 + PS417_17115 0.84 +0.3 3,738,083 - PS417_17115 0.86 -0.0 3,738,083 - PS417_17115 0.86 +1.2 3,738,276 + +0.8 3,738,809 - PS417_17120 0.42 +0.6 3,738,976 + PS417_17120 0.55 +0.0 3,738,976 + PS417_17120 0.55 +0.4 3,739,183 + PS417_17120 0.71 -0.0 3,739,184 - PS417_17120 0.71 +0.7 3,739,184 - PS417_17120 0.71 +0.7 3,739,291 + PS417_17120 0.79 +0.3 3,739,302 + PS417_17120 0.80 +0.1 3,739,681 + -0.2 3,739,801 + PS417_17125 0.17 +0.2 3,739,801 + PS417_17125 0.17 -0.2 3,739,802 - PS417_17125 0.17 +1.2 3,740,075 + PS417_17125 0.56 -0.4 3,740,140 + PS417_17125 0.65 -0.5 3,740,360 + +0.0 3,740,452 - -0.2 3,740,470 - +0.3
Or see this region's nucleotide sequence