Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_15915 and PS417_15920 overlap by 8 nucleotides PS417_15920 and PS417_15930 are separated by 98 nucleotides PS417_15930 and PS417_15935 are separated by 144 nucleotides PS417_15935 and PS417_15940 are separated by 4 nucleotides
PS417_15915: PS417_15915 - hypothetical protein, at 3,484,942 to 3,485,217
_15915
PS417_15920: PS417_15920 - TetR family transcriptional regulator, at 3,485,210 to 3,485,788
_15920
PS417_15930: PS417_15930 - thioredoxin reductase, at 3,485,887 to 3,486,771
_15930
PS417_15935: PS417_15935 - Rrf2 family transcriptional regulator, at 3,486,916 to 3,487,353
_15935
PS417_15940: PS417_15940 - mechanosensitive ion channel protein MscS, at 3,487,358 to 3,488,803
_15940
Position (kb)
3485
3486
3487 Strain fitness (log2 ratio)
-1
0
1 at 3484.926 kb on + strand at 3484.926 kb on + strand at 3484.926 kb on + strand at 3485.107 kb on + strand, within PS417_15915 at 3485.108 kb on - strand, within PS417_15915 at 3485.513 kb on - strand, within PS417_15920 at 3486.253 kb on + strand, within PS417_15930 at 3486.254 kb on - strand, within PS417_15930 at 3486.268 kb on + strand, within PS417_15930 at 3486.269 kb on - strand, within PS417_15930 at 3486.340 kb on + strand, within PS417_15930 at 3486.737 kb on + strand at 3486.764 kb on + strand at 3486.855 kb on - strand at 3487.069 kb on + strand, within PS417_15935 at 3487.070 kb on - strand at 3487.073 kb on + strand, within PS417_15935 at 3487.074 kb on - strand at 3487.149 kb on + strand, within PS417_15935 at 3487.360 kb on - strand at 3487.360 kb on - strand at 3487.534 kb on - strand, within PS417_15940 at 3487.534 kb on - strand, within PS417_15940 at 3487.690 kb on + strand, within PS417_15940 at 3487.691 kb on - strand, within PS417_15940 at 3487.691 kb on - strand, within PS417_15940
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 3,484,926 + +0.5 3,484,926 + +0.5 3,484,926 + -0.1 3,485,107 + PS417_15915 0.60 -0.2 3,485,108 - PS417_15915 0.60 -0.0 3,485,513 - PS417_15920 0.52 -0.1 3,486,253 + PS417_15930 0.41 +0.5 3,486,254 - PS417_15930 0.41 -0.1 3,486,268 + PS417_15930 0.43 +0.9 3,486,269 - PS417_15930 0.43 +0.1 3,486,340 + PS417_15930 0.51 -0.1 3,486,737 + -0.5 3,486,764 + -0.3 3,486,855 - -0.2 3,487,069 + PS417_15935 0.35 +0.0 3,487,070 - +0.4 3,487,073 + PS417_15935 0.36 +0.2 3,487,074 - -0.5 3,487,149 + PS417_15935 0.53 -0.0 3,487,360 - -0.3 3,487,360 - -0.0 3,487,534 - PS417_15940 0.12 -0.0 3,487,534 - PS417_15940 0.12 +0.1 3,487,690 + PS417_15940 0.23 -0.1 3,487,691 - PS417_15940 0.23 +0.2 3,487,691 - PS417_15940 0.23 +0.1
Or see this region's nucleotide sequence