Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14420 and PS417_14425 are separated by 34 nucleotides PS417_14425 and PS417_14430 are separated by 102 nucleotides PS417_14430 and PS417_14435 overlap by 4 nucleotides
PS417_14420: PS417_14420 - acyl-CoA dehydrogenase, at 3,151,979 to 3,153,040
_14420
PS417_14425: PS417_14425 - alkylhydroperoxidase, at 3,153,075 to 3,153,635
_14425
PS417_14430: PS417_14430 - AraC family transcriptional regulator, at 3,153,738 to 3,154,643
_14430
PS417_14435: PS417_14435 - transcriptional regulator, at 3,154,640 to 3,155,551
_14435
Position (kb)
3153
3154
3155 Strain fitness (log2 ratio)
-1
0
1 at 3152.797 kb on - strand, within PS417_14420 at 3152.933 kb on + strand, within PS417_14420 at 3152.934 kb on - strand at 3153.299 kb on - strand, within PS417_14425 at 3153.491 kb on + strand, within PS417_14425 at 3153.496 kb on + strand, within PS417_14425 at 3153.496 kb on + strand, within PS417_14425 at 3153.497 kb on - strand, within PS417_14425 at 3153.497 kb on - strand, within PS417_14425 at 3153.543 kb on - strand, within PS417_14425 at 3153.772 kb on + strand at 3153.773 kb on - strand at 3153.773 kb on - strand at 3153.773 kb on - strand at 3153.877 kb on + strand, within PS417_14430 at 3153.878 kb on - strand, within PS417_14430 at 3154.266 kb on + strand, within PS417_14430 at 3154.803 kb on - strand, within PS417_14435 at 3154.821 kb on + strand, within PS417_14435 at 3154.977 kb on + strand, within PS417_14435 at 3154.978 kb on - strand, within PS417_14435 at 3155.040 kb on + strand, within PS417_14435 at 3155.040 kb on + strand, within PS417_14435 at 3155.041 kb on - strand, within PS417_14435 at 3155.356 kb on - strand, within PS417_14435 at 3155.415 kb on - strand, within PS417_14435
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 3,152,797 - PS417_14420 0.77 +0.3 3,152,933 + PS417_14420 0.90 -0.1 3,152,934 - -0.5 3,153,299 - PS417_14425 0.40 -0.1 3,153,491 + PS417_14425 0.74 +0.7 3,153,496 + PS417_14425 0.75 +0.1 3,153,496 + PS417_14425 0.75 +0.2 3,153,497 - PS417_14425 0.75 +0.6 3,153,497 - PS417_14425 0.75 +0.6 3,153,543 - PS417_14425 0.83 -1.2 3,153,772 + +0.1 3,153,773 - -0.7 3,153,773 - +0.1 3,153,773 - -0.1 3,153,877 + PS417_14430 0.15 +0.0 3,153,878 - PS417_14430 0.15 +0.2 3,154,266 + PS417_14430 0.58 +1.1 3,154,803 - PS417_14435 0.18 -0.2 3,154,821 + PS417_14435 0.20 -0.2 3,154,977 + PS417_14435 0.37 -0.7 3,154,978 - PS417_14435 0.37 -0.0 3,155,040 + PS417_14435 0.44 +0.1 3,155,040 + PS417_14435 0.44 +0.5 3,155,041 - PS417_14435 0.44 -0.2 3,155,356 - PS417_14435 0.79 +0.0 3,155,415 - PS417_14435 0.85 +0.1
Or see this region's nucleotide sequence