Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_14135 and PS417_14140 are separated by 69 nucleotides PS417_14140 and PS417_14145 are separated by 146 nucleotides PS417_14145 and PS417_14150 are separated by 170 nucleotides PS417_14150 and PS417_14155 overlap by 4 nucleotides
PS417_14135: PS417_14135 - ABC transporter ATP-binding protein, at 3,100,497 to 3,102,119
_14135
PS417_14140: PS417_14140 - membrane protein, at 3,102,189 to 3,102,893
_14140
PS417_14145: PS417_14145 - hypothetical protein, at 3,103,040 to 3,103,393
_14145
PS417_14150: PS417_14150 - branched-chain amino acid ABC transporter, at 3,103,564 to 3,103,875
_14150
PS417_14155: PS417_14155 - branched-chain amino acid ABC transporter permease, at 3,103,872 to 3,104,567
_14155
Position (kb)
3103
3104 Strain fitness (log2 ratio)
-1
0
1 at 3102.188 kb on + strand at 3102.213 kb on + strand at 3102.214 kb on - strand at 3102.645 kb on + strand, within PS417_14140 at 3102.645 kb on + strand, within PS417_14140 at 3102.645 kb on + strand, within PS417_14140 at 3102.646 kb on - strand, within PS417_14140 at 3102.879 kb on + strand at 3102.880 kb on - strand at 3102.923 kb on - strand at 3103.029 kb on - strand at 3103.085 kb on - strand, within PS417_14145 at 3103.085 kb on - strand, within PS417_14145 at 3103.101 kb on + strand, within PS417_14145 at 3103.101 kb on + strand, within PS417_14145 at 3103.102 kb on - strand, within PS417_14145 at 3103.102 kb on - strand, within PS417_14145 at 3103.102 kb on - strand, within PS417_14145 at 3103.104 kb on + strand, within PS417_14145 at 3103.104 kb on + strand at 3103.104 kb on + strand, within PS417_14145 at 3103.105 kb on - strand, within PS417_14145 at 3103.379 kb on + strand at 3103.379 kb on + strand at 3103.379 kb on + strand at 3103.379 kb on + strand at 3103.380 kb on - strand at 3103.424 kb on + strand at 3103.425 kb on - strand at 3103.425 kb on - strand at 3103.493 kb on + strand at 3103.493 kb on + strand at 3103.494 kb on - strand at 3103.494 kb on - strand at 3103.515 kb on - strand at 3103.517 kb on + strand at 3103.673 kb on + strand, within PS417_14150 at 3103.869 kb on - strand at 3103.869 kb on - strand at 3103.869 kb on - strand at 3103.869 kb on - strand at 3103.869 kb on - strand at 3104.017 kb on - strand, within PS417_14155 at 3104.017 kb on - strand at 3104.017 kb on - strand, within PS417_14155 at 3104.149 kb on + strand, within PS417_14155 at 3104.149 kb on + strand, within PS417_14155 at 3104.149 kb on + strand, within PS417_14155 at 3104.150 kb on - strand, within PS417_14155 at 3104.176 kb on + strand, within PS417_14155 at 3104.203 kb on + strand, within PS417_14155 at 3104.203 kb on + strand, within PS417_14155
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 3,102,188 + -0.2 3,102,213 + +0.2 3,102,214 - +0.4 3,102,645 + PS417_14140 0.65 -0.5 3,102,645 + PS417_14140 0.65 -0.8 3,102,645 + PS417_14140 0.65 +0.5 3,102,646 - PS417_14140 0.65 +0.1 3,102,879 + -0.8 3,102,880 - -0.3 3,102,923 - +0.3 3,103,029 - +0.3 3,103,085 - PS417_14145 0.13 -0.4 3,103,085 - PS417_14145 0.13 -0.4 3,103,101 + PS417_14145 0.17 -0.7 3,103,101 + PS417_14145 0.17 -0.2 3,103,102 - PS417_14145 0.18 -0.7 3,103,102 - PS417_14145 0.18 -0.3 3,103,102 - PS417_14145 0.18 -0.5 3,103,104 + PS417_14145 0.18 -0.5 3,103,104 + -0.5 3,103,104 + PS417_14145 0.18 -0.2 3,103,105 - PS417_14145 0.18 +0.0 3,103,379 + -0.1 3,103,379 + +0.1 3,103,379 + -0.4 3,103,379 + -0.7 3,103,380 - -0.0 3,103,424 + -0.2 3,103,425 - -0.2 3,103,425 - -0.1 3,103,493 + +0.1 3,103,493 + -0.8 3,103,494 - +0.2 3,103,494 - -0.1 3,103,515 - +0.2 3,103,517 + -0.1 3,103,673 + PS417_14150 0.35 +0.4 3,103,869 - +0.2 3,103,869 - -0.2 3,103,869 - -0.7 3,103,869 - +0.1 3,103,869 - -0.1 3,104,017 - PS417_14155 0.21 +0.6 3,104,017 - -0.5 3,104,017 - PS417_14155 0.21 -0.6 3,104,149 + PS417_14155 0.40 -0.2 3,104,149 + PS417_14155 0.40 +0.7 3,104,149 + PS417_14155 0.40 +0.2 3,104,150 - PS417_14155 0.40 +0.0 3,104,176 + PS417_14155 0.44 +0.1 3,104,203 + PS417_14155 0.48 -0.1 3,104,203 + PS417_14155 0.48 +0.1
Or see this region's nucleotide sequence