Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01385 and PS417_01390 are separated by 62 nucleotides PS417_01390 and PS417_01395 overlap by 23 nucleotides
PS417_01385: PS417_01385 - NAD-dependent dehydratase, at 302,396 to 303,328
_01385
PS417_01390: PS417_01390 - PbsX family transcriptional regulator, at 303,391 to 306,204
_01390
PS417_01395: PS417_01395 - biofilm formation protein PelB, at 306,182 to 309,781
_01395
Position (kb) 303
304
305
306
307 Strain fitness (log2 ratio) -2
-1
0
1
2 at 302.404 kb on - strand at 302.438 kb on - strand at 302.477 kb on + strand at 302.512 kb on + strand, within PS417_01385 at 302.832 kb on - strand, within PS417_01385 at 302.859 kb on - strand, within PS417_01385 at 302.859 kb on - strand at 302.861 kb on + strand, within PS417_01385 at 302.874 kb on - strand, within PS417_01385 at 303.007 kb on + strand, within PS417_01385 at 303.008 kb on - strand, within PS417_01385 at 303.048 kb on - strand, within PS417_01385 at 303.120 kb on + strand, within PS417_01385 at 303.201 kb on - strand, within PS417_01385 at 303.245 kb on - strand at 303.326 kb on + strand at 303.326 kb on + strand at 303.326 kb on + strand at 303.354 kb on + strand at 303.354 kb on + strand at 303.355 kb on - strand at 303.362 kb on - strand at 303.386 kb on - strand at 303.495 kb on - strand at 303.643 kb on + strand at 303.644 kb on - strand at 303.817 kb on + strand, within PS417_01390 at 304.139 kb on - strand, within PS417_01390 at 304.351 kb on + strand, within PS417_01390 at 304.351 kb on + strand, within PS417_01390 at 304.352 kb on - strand, within PS417_01390 at 304.352 kb on - strand, within PS417_01390 at 304.403 kb on - strand, within PS417_01390 at 304.420 kb on + strand, within PS417_01390 at 304.421 kb on - strand, within PS417_01390 at 304.421 kb on - strand, within PS417_01390 at 304.799 kb on - strand, within PS417_01390 at 305.011 kb on + strand at 305.012 kb on - strand, within PS417_01390 at 305.136 kb on + strand, within PS417_01390 at 305.504 kb on - strand at 305.504 kb on - strand, within PS417_01390 at 305.507 kb on - strand, within PS417_01390 at 305.557 kb on + strand, within PS417_01390 at 305.558 kb on - strand, within PS417_01390 at 305.636 kb on - strand, within PS417_01390 at 305.689 kb on + strand, within PS417_01390 at 305.690 kb on - strand, within PS417_01390 at 305.738 kb on - strand, within PS417_01390 at 305.738 kb on - strand, within PS417_01390 at 305.762 kb on - strand, within PS417_01390 at 305.915 kb on - strand, within PS417_01390 at 306.202 kb on + strand at 306.203 kb on - strand at 306.338 kb on + strand at 306.338 kb on + strand at 306.339 kb on - strand at 306.339 kb on - strand at 306.349 kb on - strand at 306.482 kb on + strand at 306.694 kb on + strand, within PS417_01395 at 306.707 kb on - strand, within PS417_01395 at 306.764 kb on + strand, within PS417_01395 at 306.764 kb on + strand, within PS417_01395 at 306.765 kb on - strand, within PS417_01395 at 306.765 kb on - strand, within PS417_01395 at 306.775 kb on + strand, within PS417_01395 at 306.775 kb on + strand, within PS417_01395 at 306.776 kb on - strand, within PS417_01395 at 307.047 kb on + strand, within PS417_01395 at 307.047 kb on + strand, within PS417_01395 at 307.047 kb on + strand, within PS417_01395 at 307.048 kb on - strand, within PS417_01395 at 307.048 kb on - strand, within PS417_01395 at 307.048 kb on - strand, within PS417_01395 at 307.057 kb on + strand, within PS417_01395
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 302,404 - +2.2 302,438 - -0.1 302,477 + +0.4 302,512 + PS417_01385 0.12 +0.5 302,832 - PS417_01385 0.47 +1.7 302,859 - PS417_01385 0.50 -0.1 302,859 - +0.6 302,861 + PS417_01385 0.50 -0.4 302,874 - PS417_01385 0.51 +0.6 303,007 + PS417_01385 0.65 -0.4 303,008 - PS417_01385 0.66 +0.9 303,048 - PS417_01385 0.70 -0.3 303,120 + PS417_01385 0.78 -0.4 303,201 - PS417_01385 0.86 +0.4 303,245 - +0.1 303,326 + +0.9 303,326 + +0.2 303,326 + -0.4 303,354 + -2.0 303,354 + -0.3 303,355 - +0.3 303,362 - -0.3 303,386 - +1.2 303,495 - +0.1 303,643 + +0.4 303,644 - +0.0 303,817 + PS417_01390 0.15 +0.5 304,139 - PS417_01390 0.27 +0.6 304,351 + PS417_01390 0.34 +0.2 304,351 + PS417_01390 0.34 +0.7 304,352 - PS417_01390 0.34 +0.2 304,352 - PS417_01390 0.34 +0.9 304,403 - PS417_01390 0.36 +0.7 304,420 + PS417_01390 0.37 -0.1 304,421 - PS417_01390 0.37 -0.5 304,421 - PS417_01390 0.37 +1.3 304,799 - PS417_01390 0.50 +0.2 305,011 + +0.4 305,012 - PS417_01390 0.58 +0.4 305,136 + PS417_01390 0.62 -0.7 305,504 - +2.0 305,504 - PS417_01390 0.75 +1.0 305,507 - PS417_01390 0.75 +0.7 305,557 + PS417_01390 0.77 +0.1 305,558 - PS417_01390 0.77 +0.2 305,636 - PS417_01390 0.80 +0.2 305,689 + PS417_01390 0.82 +0.1 305,690 - PS417_01390 0.82 +0.0 305,738 - PS417_01390 0.83 +0.6 305,738 - PS417_01390 0.83 +0.1 305,762 - PS417_01390 0.84 +0.1 305,915 - PS417_01390 0.90 +0.1 306,202 + +0.0 306,203 - -0.0 306,338 + +0.2 306,338 + +0.1 306,339 - -1.4 306,339 - +0.0 306,349 - +0.5 306,482 + +0.0 306,694 + PS417_01395 0.14 +0.7 306,707 - PS417_01395 0.15 -0.1 306,764 + PS417_01395 0.16 -0.0 306,764 + PS417_01395 0.16 +0.1 306,765 - PS417_01395 0.16 -0.6 306,765 - PS417_01395 0.16 -0.6 306,775 + PS417_01395 0.16 +0.5 306,775 + PS417_01395 0.16 +0.2 306,776 - PS417_01395 0.17 -0.2 307,047 + PS417_01395 0.24 +0.1 307,047 + PS417_01395 0.24 +0.0 307,047 + PS417_01395 0.24 +0.5 307,048 - PS417_01395 0.24 +0.3 307,048 - PS417_01395 0.24 +0.1 307,048 - PS417_01395 0.24 -0.2 307,057 + PS417_01395 0.24 -0.1
Or see this region's nucleotide sequence