Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01135 and PS417_01140 are separated by 14 nucleotides PS417_01140 and PS417_01145 overlap by 4 nucleotides PS417_01145 and PS417_01150 are separated by 16 nucleotides
PS417_01135: PS417_01135 - taurine dioxygenase, at 249,832 to 250,668
_01135
PS417_01140: PS417_01140 - taurine ABC transporter permease, at 250,683 to 251,519
_01140
PS417_01145: PS417_01145 - taurine transporter ATP-binding subunit, at 251,516 to 252,295
_01145
PS417_01150: PS417_01150 - taurine ABC transporter substrate-binding protein, at 252,312 to 253,289
_01150
Position (kb) 251
252
253 Strain fitness (log2 ratio) -1
0
1 at 250.651 kb on - strand at 250.668 kb on + strand at 250.669 kb on - strand at 250.771 kb on - strand, within PS417_01140 at 250.771 kb on - strand, within PS417_01140 at 251.146 kb on + strand, within PS417_01140 at 251.623 kb on - strand, within PS417_01145 at 252.237 kb on - strand at 252.284 kb on + strand at 252.284 kb on + strand at 252.303 kb on - strand at 252.340 kb on + strand at 252.340 kb on + strand at 252.341 kb on - strand at 252.341 kb on - strand at 252.349 kb on + strand at 252.419 kb on + strand, within PS417_01150 at 252.584 kb on - strand, within PS417_01150 at 252.757 kb on + strand, within PS417_01150 at 252.758 kb on - strand at 252.758 kb on - strand, within PS417_01150 at 252.758 kb on - strand, within PS417_01150 at 252.825 kb on + strand, within PS417_01150 at 253.185 kb on + strand, within PS417_01150
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 250,651 - +0.4 250,668 + +0.3 250,669 - +0.1 250,771 - PS417_01140 0.11 +0.1 250,771 - PS417_01140 0.11 +0.1 251,146 + PS417_01140 0.55 +0.2 251,623 - PS417_01145 0.14 +0.5 252,237 - -0.8 252,284 + -0.5 252,284 + +0.1 252,303 - +0.8 252,340 + +0.3 252,340 + +0.0 252,341 - -0.0 252,341 - -0.2 252,349 + +1.0 252,419 + PS417_01150 0.11 -0.1 252,584 - PS417_01150 0.28 -0.2 252,757 + PS417_01150 0.46 +0.2 252,758 - -0.8 252,758 - PS417_01150 0.46 -0.2 252,758 - PS417_01150 0.46 -0.0 252,825 + PS417_01150 0.52 +0.3 253,185 + PS417_01150 0.89 -0.3
Or see this region's nucleotide sequence