Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11475 and PS417_11480 are separated by 289 nucleotides PS417_11480 and PS417_11485 are separated by 146 nucleotides PS417_11485 and PS417_11495 are separated by 293 nucleotides
PS417_11475: PS417_11475 - membrane protein, at 2,469,931 to 2,470,383
_11475
PS417_11480: PS417_11480 - hypothetical protein, at 2,470,673 to 2,470,945
_11480
PS417_11485: PS417_11485 - AraC family transcriptional regulator, at 2,471,092 to 2,471,856
_11485
PS417_11495: PS417_11495 - oxidoreductase, at 2,472,150 to 2,473,109
_11495
Position (kb)
2471
2472 Strain fitness (log2 ratio)
-1
0
1 at 2470.168 kb on + strand, within PS417_11475 at 2470.168 kb on + strand, within PS417_11475 at 2470.169 kb on - strand, within PS417_11475 at 2470.169 kb on - strand, within PS417_11475 at 2470.675 kb on - strand at 2470.703 kb on - strand, within PS417_11480 at 2470.730 kb on + strand at 2470.731 kb on - strand, within PS417_11480 at 2470.788 kb on - strand, within PS417_11480 at 2470.902 kb on + strand, within PS417_11480 at 2470.998 kb on + strand at 2470.999 kb on - strand at 2471.057 kb on - strand at 2471.159 kb on - strand at 2471.173 kb on + strand, within PS417_11485 at 2471.347 kb on + strand, within PS417_11485 at 2471.347 kb on + strand, within PS417_11485 at 2471.845 kb on + strand at 2471.910 kb on + strand at 2471.911 kb on - strand at 2472.441 kb on + strand, within PS417_11495 at 2472.686 kb on - strand, within PS417_11495
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 2,470,168 + PS417_11475 0.52 +0.4 2,470,168 + PS417_11475 0.52 +0.6 2,470,169 - PS417_11475 0.53 +0.0 2,470,169 - PS417_11475 0.53 -0.8 2,470,675 - -0.1 2,470,703 - PS417_11480 0.11 -0.1 2,470,730 + -1.5 2,470,731 - PS417_11480 0.21 +0.2 2,470,788 - PS417_11480 0.42 +0.1 2,470,902 + PS417_11480 0.84 +0.1 2,470,998 + +0.9 2,470,999 - +0.0 2,471,057 - +0.0 2,471,159 - +0.8 2,471,173 + PS417_11485 0.11 -0.2 2,471,347 + PS417_11485 0.33 +0.0 2,471,347 + PS417_11485 0.33 -1.5 2,471,845 + -0.0 2,471,910 + -0.2 2,471,911 - -0.2 2,472,441 + PS417_11495 0.30 -0.5 2,472,686 - PS417_11495 0.56 +0.3
Or see this region's nucleotide sequence