Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07650 and PS417_07655 are separated by 42 nucleotides PS417_07655 and PS417_07660 are separated by 46 nucleotides PS417_07660 and PS417_07665 are separated by 94 nucleotides
PS417_07650: PS417_07650 - pyridine nucleotide-disulfide oxidoreductase, at 1,686,850 to 1,688,244
_07650
PS417_07655: PS417_07655 - glycerophosphodiester phosphodiesterase, at 1,688,287 to 1,689,009
_07655
PS417_07660: PS417_07660 - pilus assembly protein PilZ, at 1,689,056 to 1,689,640
_07660
PS417_07665: PS417_07665 - cell division protein FtsX, at 1,689,735 to 1,690,985
_07665
Position (kb)
1688
1689
1690 Strain fitness (log2 ratio)
-1
0
1
2 at 1688.340 kb on + strand at 1688.345 kb on - strand at 1688.484 kb on - strand, within PS417_07655 at 1688.484 kb on - strand, within PS417_07655 at 1688.814 kb on - strand at 1688.920 kb on + strand, within PS417_07655 at 1688.996 kb on + strand at 1688.996 kb on + strand at 1688.997 kb on - strand at 1688.997 kb on - strand at 1688.997 kb on - strand at 1688.997 kb on - strand at 1689.124 kb on - strand, within PS417_07660 at 1689.606 kb on + strand at 1689.606 kb on + strand at 1689.674 kb on + strand at 1689.674 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 1,688,340 + +0.2 1,688,345 - +0.0 1,688,484 - PS417_07655 0.27 +1.5 1,688,484 - PS417_07655 0.27 +0.3 1,688,814 - +2.2 1,688,920 + PS417_07655 0.88 +0.1 1,688,996 + +0.5 1,688,996 + -1.0 1,688,997 - +0.1 1,688,997 - +0.2 1,688,997 - -0.1 1,688,997 - +0.4 1,689,124 - PS417_07660 0.12 +0.8 1,689,606 + +0.9 1,689,606 + -0.1 1,689,674 + -0.0 1,689,674 + +1.1
Or see this region's nucleotide sequence