Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02870 and PS417_02875 are separated by 93 nucleotides PS417_02875 and PS417_02880 are separated by 11 nucleotides PS417_02880 and PS417_02885 are separated by 47 nucleotides PS417_02885 and PS417_02890 are separated by 262 nucleotides
PS417_02870: PS417_02870 - TonB-dependent receptor, at 656,099 to 658,168
_02870
PS417_02875: PS417_02875 - hypothetical protein, at 658,262 to 658,651
_02875
PS417_02880: PS417_02880 - copper chaperone, at 658,663 to 659,145
_02880
PS417_02885: PS417_02885 - hypothetical protein, at 659,193 to 659,588
_02885
PS417_02890: PS417_02890 - cobalt-precorrin-6X reductase, at 659,851 to 660,570
_02890
Position (kb) 658
659
660 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 657.777 kb on + strand, within PS417_02870 at 657.778 kb on - strand, within PS417_02870 at 657.778 kb on - strand, within PS417_02870 at 657.795 kb on + strand, within PS417_02870 at 658.133 kb on - strand at 658.133 kb on - strand at 658.135 kb on - strand at 658.191 kb on + strand at 658.246 kb on + strand at 658.247 kb on - strand at 658.517 kb on - strand, within PS417_02875 at 658.664 kb on + strand at 658.877 kb on - strand, within PS417_02880 at 659.076 kb on - strand, within PS417_02880 at 659.285 kb on + strand, within PS417_02885 at 659.303 kb on - strand, within PS417_02885 at 659.303 kb on - strand, within PS417_02885 at 659.303 kb on - strand, within PS417_02885 at 659.470 kb on - strand, within PS417_02885 at 659.629 kb on - strand at 659.651 kb on - strand at 659.651 kb on - strand at 659.651 kb on - strand at 659.743 kb on - strand at 659.743 kb on - strand at 659.743 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 657,777 + PS417_02870 0.81 -1.8 657,778 - PS417_02870 0.81 +2.8 657,778 - PS417_02870 0.81 -2.4 657,795 + PS417_02870 0.82 +1.5 658,133 - +1.5 658,133 - +0.6 658,135 - +1.8 658,191 + +0.1 658,246 + +1.5 658,247 - -1.7 658,517 - PS417_02875 0.65 -0.8 658,664 + +0.5 658,877 - PS417_02880 0.44 +1.5 659,076 - PS417_02880 0.86 -0.5 659,285 + PS417_02885 0.23 +1.5 659,303 - PS417_02885 0.28 -0.5 659,303 - PS417_02885 0.28 -0.5 659,303 - PS417_02885 0.28 +0.2 659,470 - PS417_02885 0.70 -0.3 659,629 - -0.3 659,651 - +0.4 659,651 - +0.2 659,651 - -0.3 659,743 - -0.8 659,743 - +0.5 659,743 - -0.4
Or see this region's nucleotide sequence