Strain Fitness in Pseudomonas simiae WCS417 around PS417_28270

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_28265 and PS417_28270 are separated by 117 nucleotidesPS417_28270 and PS417_28275 overlap by 7 nucleotides PS417_28265: PS417_28265 - pyridoxamine kinase, at 6,105,159 to 6,106,031 _28265 PS417_28270: PS417_28270 - hypothetical protein, at 6,106,149 to 6,106,544 _28270 PS417_28275: PS417_28275 - cobalamin biosynthesis protein CobW, at 6,106,538 to 6,107,500 _28275 Position (kb) 6106 6107Strain fitness (log2 ratio) -2 -1 0 1at 6105.160 kb on + strandat 6105.160 kb on + strandat 6105.160 kb on + strandat 6105.160 kb on + strandat 6105.162 kb on + strandat 6105.222 kb on + strandat 6105.222 kb on + strandat 6105.701 kb on - strand, within PS417_28265at 6105.701 kb on - strand, within PS417_28265at 6106.075 kb on - strandat 6106.082 kb on + strandat 6106.313 kb on - strand, within PS417_28270at 6106.313 kb on - strand, within PS417_28270at 6106.360 kb on - strand, within PS417_28270at 6106.501 kb on + strand, within PS417_28270at 6106.944 kb on - strand, within PS417_28275at 6106.966 kb on - strandat 6107.243 kb on - strand, within PS417_28275at 6107.265 kb on - strand, within PS417_28275at 6107.538 kb on + strandat 6107.539 kb on - strandat 6107.539 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
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6,105,160 + -0.3
6,105,160 + +0.6
6,105,160 + +0.0
6,105,160 + +0.1
6,105,162 + +0.0
6,105,222 + -0.0
6,105,222 + +0.2
6,105,701 - PS417_28265 0.62 +0.1
6,105,701 - PS417_28265 0.62 -0.4
6,106,075 - +0.1
6,106,082 + +0.6
6,106,313 - PS417_28270 0.41 -0.4
6,106,313 - PS417_28270 0.41 -0.4
6,106,360 - PS417_28270 0.53 -0.6
6,106,501 + PS417_28270 0.89 -0.7
6,106,944 - PS417_28275 0.42 -0.1
6,106,966 - -2.1
6,107,243 - PS417_28275 0.73 -0.1
6,107,265 - PS417_28275 0.75 -0.8
6,107,538 + +0.2
6,107,539 - -0.3
6,107,539 - +0.2

Or see this region's nucleotide sequence