Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27495 and PS417_27500 are separated by 231 nucleotides PS417_27500 and PS417_27505 are separated by 53 nucleotides PS417_27505 and PS417_27510 are separated by 209 nucleotides PS417_27510 and PS417_27515 are separated by 236 nucleotides
PS417_27495: PS417_27495 - hypothetical protein, at 5,933,571 to 5,933,816
_27495
PS417_27500: PS417_27500 - peptidylprolyl isomerase, at 5,934,048 to 5,934,725
_27500
PS417_27505: PS417_27505 - transcriptional regulator, at 5,934,779 to 5,935,237
_27505
PS417_27510: PS417_27510 - disulfide bond formation protein B, at 5,935,447 to 5,935,968
_27510
PS417_27515: PS417_27515 - heme biosynthesis protein HemY, at 5,936,205 to 5,937,446
_27515
Position (kb)
5934
5935
5936 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5933.815 kb on - strand at 5933.815 kb on - strand at 5933.831 kb on + strand at 5933.831 kb on + strand at 5933.832 kb on - strand at 5933.832 kb on - strand at 5933.861 kb on - strand at 5933.889 kb on + strand at 5933.889 kb on + strand at 5933.889 kb on + strand at 5933.889 kb on + strand at 5933.892 kb on + strand at 5933.892 kb on + strand at 5933.892 kb on + strand at 5933.892 kb on + strand at 5933.893 kb on - strand at 5933.893 kb on - strand at 5933.976 kb on - strand at 5933.999 kb on - strand at 5933.999 kb on - strand at 5934.016 kb on + strand at 5934.233 kb on - strand, within PS417_27500 at 5934.489 kb on + strand, within PS417_27500 at 5934.489 kb on + strand, within PS417_27500 at 5934.489 kb on + strand, within PS417_27500 at 5934.490 kb on - strand, within PS417_27500 at 5934.642 kb on + strand, within PS417_27500 at 5934.643 kb on - strand, within PS417_27500 at 5934.707 kb on - strand at 5934.707 kb on - strand at 5934.805 kb on + strand at 5934.805 kb on + strand at 5934.933 kb on - strand, within PS417_27505 at 5934.933 kb on - strand, within PS417_27505 at 5934.933 kb on - strand, within PS417_27505 at 5934.964 kb on - strand, within PS417_27505 at 5934.964 kb on - strand, within PS417_27505 at 5935.059 kb on + strand, within PS417_27505 at 5935.060 kb on - strand, within PS417_27505 at 5935.060 kb on - strand, within PS417_27505 at 5935.254 kb on + strand at 5935.254 kb on + strand at 5935.255 kb on - strand at 5935.360 kb on + strand at 5935.361 kb on - strand at 5935.361 kb on - strand at 5935.407 kb on + strand at 5935.418 kb on - strand at 5935.428 kb on + strand at 5935.649 kb on + strand, within PS417_27510 at 5935.649 kb on + strand, within PS417_27510 at 5935.650 kb on - strand, within PS417_27510 at 5935.650 kb on - strand, within PS417_27510 at 5935.763 kb on + strand, within PS417_27510 at 5935.764 kb on - strand, within PS417_27510 at 5935.893 kb on - strand, within PS417_27510 at 5935.991 kb on + strand at 5935.992 kb on - strand at 5936.030 kb on + strand at 5936.030 kb on + strand at 5936.030 kb on + strand at 5936.031 kb on - strand at 5936.031 kb on - strand at 5936.071 kb on - strand at 5936.130 kb on + strand at 5936.130 kb on + strand at 5936.131 kb on - strand at 5936.131 kb on - strand at 5936.193 kb on + strand at 5936.193 kb on + strand at 5936.194 kb on - strand at 5936.201 kb on + strand at 5936.202 kb on - strand at 5936.202 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 5,933,815 - +0.1 5,933,815 - -0.1 5,933,831 + -0.4 5,933,831 + -0.2 5,933,832 - -0.1 5,933,832 - -0.1 5,933,861 - -0.7 5,933,889 + +0.2 5,933,889 + -0.1 5,933,889 + -0.0 5,933,889 + -0.1 5,933,892 + +0.0 5,933,892 + -0.3 5,933,892 + +0.1 5,933,892 + -0.5 5,933,893 - +0.1 5,933,893 - -0.6 5,933,976 - -0.9 5,933,999 - +0.5 5,933,999 - -0.6 5,934,016 + -1.2 5,934,233 - PS417_27500 0.27 +1.2 5,934,489 + PS417_27500 0.65 +0.0 5,934,489 + PS417_27500 0.65 -0.3 5,934,489 + PS417_27500 0.65 +0.3 5,934,490 - PS417_27500 0.65 -0.2 5,934,642 + PS417_27500 0.88 +2.0 5,934,643 - PS417_27500 0.88 +0.2 5,934,707 - -0.8 5,934,707 - -0.5 5,934,805 + -0.3 5,934,805 + -0.3 5,934,933 - PS417_27505 0.34 -1.3 5,934,933 - PS417_27505 0.34 -0.4 5,934,933 - PS417_27505 0.34 +0.1 5,934,964 - PS417_27505 0.40 -2.1 5,934,964 - PS417_27505 0.40 +0.0 5,935,059 + PS417_27505 0.61 -1.8 5,935,060 - PS417_27505 0.61 -0.0 5,935,060 - PS417_27505 0.61 +0.2 5,935,254 + +0.4 5,935,254 + +0.1 5,935,255 - +0.6 5,935,360 + -0.4 5,935,361 - +0.0 5,935,361 - -1.0 5,935,407 + +0.5 5,935,418 - +1.0 5,935,428 + -0.0 5,935,649 + PS417_27510 0.39 -0.3 5,935,649 + PS417_27510 0.39 -1.2 5,935,650 - PS417_27510 0.39 -0.5 5,935,650 - PS417_27510 0.39 +0.1 5,935,763 + PS417_27510 0.61 -0.0 5,935,764 - PS417_27510 0.61 -0.9 5,935,893 - PS417_27510 0.85 +0.2 5,935,991 + -0.3 5,935,992 - -0.4 5,936,030 + -0.1 5,936,030 + -0.1 5,936,030 + -0.9 5,936,031 - +1.2 5,936,031 - -0.8 5,936,071 - +1.2 5,936,130 + +0.3 5,936,130 + -0.5 5,936,131 - +0.3 5,936,131 - +0.5 5,936,193 + +0.1 5,936,193 + -0.2 5,936,194 - +0.2 5,936,201 + -0.2 5,936,202 - +1.2 5,936,202 - +0.3
Or see this region's nucleotide sequence