Strain Fitness in Pseudomonas simiae WCS417 around PS417_27000

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_26990 and PS417_26995 are separated by 143 nucleotidesPS417_26995 and PS417_27000 are separated by 112 nucleotidesPS417_27000 and PS417_27005 are separated by 0 nucleotidesPS417_27005 and PS417_27010 are separated by 41 nucleotides PS417_26990: PS417_26990 - RNA pyrophosphohydrolase, at 5,830,126 to 5,830,605 _26990 PS417_26995: PS417_26995 - phosphoserine phosphatase, at 5,830,749 to 5,831,405 _26995 PS417_27000: PS417_27000 - membrane protein, at 5,831,518 to 5,831,985 _27000 PS417_27005: PS417_27005 - membrane protein, at 5,831,986 to 5,832,399 _27005 PS417_27010: PS417_27010 - threonine dehydratase, at 5,832,441 to 5,833,955 _27010 Position (kb) 5831 5832Strain fitness (log2 ratio) -2 -1 0 1at 5830.676 kb on + strandat 5830.676 kb on + strandat 5830.676 kb on + strandat 5830.677 kb on - strandat 5830.681 kb on + strandat 5830.682 kb on - strandat 5830.682 kb on - strandat 5830.706 kb on + strandat 5830.706 kb on + strandat 5830.819 kb on - strand, within PS417_26995at 5830.986 kb on + strand, within PS417_26995at 5830.986 kb on + strand, within PS417_26995at 5830.987 kb on - strand, within PS417_26995at 5830.987 kb on - strand, within PS417_26995at 5830.988 kb on + strand, within PS417_26995at 5830.988 kb on + strand, within PS417_26995at 5830.988 kb on + strand, within PS417_26995at 5830.988 kb on + strand, within PS417_26995at 5830.989 kb on - strand, within PS417_26995at 5830.989 kb on - strand, within PS417_26995at 5831.271 kb on + strand, within PS417_26995at 5831.271 kb on + strand, within PS417_26995at 5831.271 kb on + strand, within PS417_26995at 5831.272 kb on - strand, within PS417_26995at 5831.272 kb on - strand, within PS417_26995at 5831.277 kb on + strand, within PS417_26995at 5831.277 kb on + strand, within PS417_26995at 5831.519 kb on + strandat 5831.519 kb on + strandat 5831.523 kb on + strandat 5831.523 kb on + strandat 5831.523 kb on + strandat 5831.523 kb on + strandat 5831.524 kb on - strandat 5831.524 kb on - strandat 5831.524 kb on - strandat 5831.524 kb on - strandat 5831.524 kb on - strandat 5831.603 kb on + strand, within PS417_27000at 5831.603 kb on + strand, within PS417_27000at 5831.718 kb on - strand, within PS417_27000at 5831.906 kb on + strand, within PS417_27000at 5831.907 kb on - strand, within PS417_27000at 5831.952 kb on + strandat 5831.952 kb on + strandat 5831.952 kb on + strandat 5831.953 kb on - strandat 5831.953 kb on - strandat 5831.975 kb on + strandat 5831.976 kb on - strandat 5831.988 kb on - strandat 5832.072 kb on + strand, within PS417_27005at 5832.435 kb on - strandat 5832.461 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
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5,830,676 + +0.0
5,830,676 + -1.2
5,830,676 + +0.6
5,830,677 - -0.8
5,830,681 + +0.3
5,830,682 - -0.2
5,830,682 - -0.4
5,830,706 + -0.6
5,830,706 + -0.6
5,830,819 - PS417_26995 0.11 -0.6
5,830,986 + PS417_26995 0.36 +0.5
5,830,986 + PS417_26995 0.36 +0.2
5,830,987 - PS417_26995 0.36 -0.1
5,830,987 - PS417_26995 0.36 +0.2
5,830,988 + PS417_26995 0.36 -0.1
5,830,988 + PS417_26995 0.36 -0.2
5,830,988 + PS417_26995 0.36 -0.0
5,830,988 + PS417_26995 0.36 -0.4
5,830,989 - PS417_26995 0.37 -0.0
5,830,989 - PS417_26995 0.37 -0.6
5,831,271 + PS417_26995 0.79 +0.0
5,831,271 + PS417_26995 0.79 -0.1
5,831,271 + PS417_26995 0.79 -0.3
5,831,272 - PS417_26995 0.80 -0.2
5,831,272 - PS417_26995 0.80 -0.4
5,831,277 + PS417_26995 0.80 -0.8
5,831,277 + PS417_26995 0.80 -0.6
5,831,519 + -1.9
5,831,519 + -2.3
5,831,523 + -0.3
5,831,523 + +0.8
5,831,523 + +0.1
5,831,523 + +0.0
5,831,524 - +0.3
5,831,524 - +0.1
5,831,524 - -0.2
5,831,524 - -0.2
5,831,524 - +0.6
5,831,603 + PS417_27000 0.18 -0.6
5,831,603 + PS417_27000 0.18 -0.0
5,831,718 - PS417_27000 0.43 -1.9
5,831,906 + PS417_27000 0.83 -0.6
5,831,907 - PS417_27000 0.83 -0.4
5,831,952 + -0.3
5,831,952 + -0.3
5,831,952 + -0.0
5,831,953 - -1.0
5,831,953 - +0.0
5,831,975 + +1.4
5,831,976 - -0.0
5,831,988 - -1.2
5,832,072 + PS417_27005 0.21 -0.1
5,832,435 - -0.3
5,832,461 - -0.1

Or see this region's nucleotide sequence