Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26720 and PS417_26725 are separated by 57 nucleotides PS417_26725 and PS417_26730 are separated by 34 nucleotides PS417_26730 and PS417_26735 are separated by 9 nucleotides
PS417_26720: PS417_26720 - twitching motility protein PilT, at 5,774,718 to 5,775,752
_26720
PS417_26725: PS417_26725 - hypothetical protein, at 5,775,810 to 5,776,496
_26725
PS417_26730: PS417_26730 - pyrroline-5-carboxylate reductase, at 5,776,531 to 5,777,349
_26730
PS417_26735: PS417_26735 - membrane protein, at 5,777,359 to 5,777,949
_26735
Position (kb) 5775
5776
5777 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5775.261 kb on - strand, within PS417_26720 at 5775.411 kb on + strand, within PS417_26720 at 5775.411 kb on + strand, within PS417_26720 at 5775.412 kb on - strand, within PS417_26720 at 5775.412 kb on - strand, within PS417_26720 at 5775.461 kb on + strand, within PS417_26720 at 5775.603 kb on + strand, within PS417_26720 at 5775.603 kb on + strand, within PS417_26720 at 5775.604 kb on - strand, within PS417_26720 at 5775.604 kb on - strand, within PS417_26720 at 5775.736 kb on - strand at 5775.949 kb on - strand, within PS417_26725 at 5775.949 kb on - strand, within PS417_26725 at 5775.949 kb on - strand, within PS417_26725 at 5775.949 kb on - strand, within PS417_26725 at 5775.981 kb on + strand, within PS417_26725 at 5775.981 kb on + strand, within PS417_26725 at 5775.981 kb on + strand, within PS417_26725 at 5775.982 kb on - strand, within PS417_26725 at 5776.015 kb on + strand, within PS417_26725 at 5776.545 kb on + strand at 5776.733 kb on + strand, within PS417_26730 at 5777.062 kb on + strand, within PS417_26730 at 5777.347 kb on + strand at 5777.347 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 5,775,261 - PS417_26720 0.52 -1.5 5,775,411 + PS417_26720 0.67 -0.2 5,775,411 + PS417_26720 0.67 +0.5 5,775,412 - PS417_26720 0.67 -0.1 5,775,412 - PS417_26720 0.67 +0.6 5,775,461 + PS417_26720 0.72 -0.0 5,775,603 + PS417_26720 0.86 +0.4 5,775,603 + PS417_26720 0.86 -0.4 5,775,604 - PS417_26720 0.86 -0.7 5,775,604 - PS417_26720 0.86 -0.3 5,775,736 - -1.6 5,775,949 - PS417_26725 0.20 +1.1 5,775,949 - PS417_26725 0.20 -0.2 5,775,949 - PS417_26725 0.20 +0.2 5,775,949 - PS417_26725 0.20 -0.8 5,775,981 + PS417_26725 0.25 -0.4 5,775,981 + PS417_26725 0.25 -0.9 5,775,981 + PS417_26725 0.25 -0.9 5,775,982 - PS417_26725 0.25 -0.5 5,776,015 + PS417_26725 0.30 -0.8 5,776,545 + -0.4 5,776,733 + PS417_26730 0.25 -1.0 5,777,062 + PS417_26730 0.65 -2.0 5,777,347 + -1.2 5,777,347 + +1.9
Or see this region's nucleotide sequence